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Response of Spring Diatoms to CO2 Availability in the Western North Pacific as Determined by Next-Generation Sequencing

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Title: Response of Spring Diatoms to CO2 Availability in the Western North Pacific as Determined by Next-Generation Sequencing
Authors: Endo, Hisashi Browse this author
Sugie, Koji Browse this author
Yoshimura, Takeshi Browse this author →KAKEN DB
Suzuki, Koji Browse this author →KAKEN DB
Issue Date: 29-Apr-2016
Publisher: PLOS
Journal Title: PLoS ONE
Volume: 11
Issue: 4
Start Page: e0154291
Publisher DOI: 10.1371/journal.pone.0154291
Abstract: Next-generation sequencing (NGS) technologies have enabled us to determine phytoplankton community compositions at high resolution. However, few studies have adopted this approach to assess the responses of natural phytoplankton communities to environmental change. Here, we report the impact of different CO2 levels on spring diatoms in the Oyashio region of the western North Pacific as estimated by NGS of the diatom-specific rbcL gene (DNA), which encodes the large subunit of RubisCO. We also examined the abundance and composition of rbcL transcripts (cDNA) in diatoms to assess their physiological responses to changing CO2 levels. A short-term (3-day) incubation experiment was carried out on-deck using surface Oyashio waters under different pCO(2) levels (180, 350, 750, and 1000 mu atm) in May 2011. During the incubation, the transcript abundance of the diatom-specific rbcL gene decreased with an increase in seawater pCO(2) levels. These results suggest that CO2 fixation capacity of diatoms decreased rapidly under elevated CO2 levels. In the high CO2 treatments (750 and 1000 mu atm), diversity of diatom-specific rbcL gene and its transcripts decreased relative to the control treatment (350 mu atm), as well as contributions of Chaetocerataceae, Thalassiosiraceae, and Fragilariaceae to the total population, but the contributions of Bacillariaceae increased. In the low CO2 treatment, contributions of Bacillariaceae also increased together with other eukaryotes. These suggest that changes in CO2 levels can alter the community composition of spring diatoms in the Oyashio region. Overall, the NGS technology provided us a deeper understanding of the response of diatoms to changes in CO2 levels in terms of their community composition, diversity, and photosynthetic physiology.
Rights: http://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/64646
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 遠藤 寿

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