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Beetroot betalain inhibits peroxynitrite-mediated tyrosine nitration and DNA strand cleavage

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Title: Beetroot betalain inhibits peroxynitrite-mediated tyrosine nitration and DNA strand cleavage
Authors: Sakihama, Yasuko Browse this author →KAKEN DB
Maeda, Makiko Browse this author
Hashimoto, Makoto Browse this author →KAKEN DB
Tahara, Satoshi Browse this author →KAKEN DB
Hashidoko, Yasuyuki Browse this author →KAKEN DB
Keywords: betanin
nitrosative stress
plant pigment
reactive nitrogen species
reactive oxygen species
Issue Date: Jan-2012
Publisher: Taylor & Francis
Journal Title: Free Radical Research
Volume: 46
Issue: 1
Start Page: 93
End Page: 99
Publisher DOI: 10.3109/10715762.2011.641157
PMID: 22087762
Abstract: Two major betalains, red-purple betacyanins and yellow betaxanthins, were isolated from red beetroots (Beta vulgaris L.), and their peroxynitrite (ONOO-) scavenging capacity was investigated. Apparent colours of the betalains were bleached by the addition of ONOO-, and the absorbance decreases were suppressed in the presence of glutathione, a ONOO- scavenger. After bleaching, a new absorption maximum was observed at 350 nm in the spectrum of the resulting reaction mixture. New peaks were detected from HPLC analysis of the reaction products of betanin, a representative constituent of red beetroot betacyanins, treated with ONOO- monitoring at 350 nm, and the intensity of the major peak was positively correlated with ONOO- concentration. Betanin inhibited the ONOO- (0.5 mM)-dependent nitration of tyrosine (0.1 mM). Additionally, the IC50 value of betanin (19.2 μM) was lower than that of ascorbate (79.6 μM). The presence of betanin (0.05-1.0 μM) also inhibited ONOO- (0.5 mM)-dependent DNA strand cleavage in a concentration-dependent manner. These results suggest that betalains can protect cells from nitrosative stress in addition to protecting them from oxidative stresses.
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 崎浜 靖子

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