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Hokkaido University Collection of Scholarly and Academic Papers >
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Beetroot betalain inhibits peroxynitrite-mediated tyrosine nitration and DNA strand cleavage
| Title: | Beetroot betalain inhibits peroxynitrite-mediated tyrosine nitration and DNA strand cleavage |
| Authors: | Sakihama, Yasuko Browse this author →KAKEN DB | | Maeda, Makiko Browse this author | | Hashimoto, Makoto Browse this author →KAKEN DB | | Tahara, Satoshi Browse this author →KAKEN DB | | Hashidoko, Yasuyuki Browse this author →KAKEN DB |
| Keywords: | betanin | | nitrosative stress | | plant pigment | | reactive nitrogen species | | reactive oxygen species |
| Issue Date: | Jan-2012 |
| Publisher: | Taylor & Francis |
| Journal Title: | Free Radical Research |
| Volume: | 46 |
| Issue: | 1 |
| Start Page: | 93 |
| End Page: | 99 |
| Publisher DOI: | 10.3109/10715762.2011.641157 |
| PMID: | 22087762 |
| Abstract: | Two major betalains, red-purple betacyanins and yellow betaxanthins, were isolated from red beetroots (Beta vulgaris L.), and their peroxynitrite (ONOO-) scavenging capacity was investigated. Apparent colours of the betalains were bleached by the addition of ONOO-, and the absorbance decreases were suppressed in the presence of glutathione, a ONOO- scavenger. After bleaching, a new absorption maximum was observed at 350 nm in the spectrum of the resulting reaction mixture. New peaks were detected from HPLC analysis of the reaction products of betanin, a representative constituent of red beetroot betacyanins, treated with ONOO- monitoring at 350 nm, and the intensity of the major peak was positively correlated with ONOO- concentration. Betanin inhibited the ONOO- (0.5 mM)-dependent nitration of tyrosine (0.1 mM). Additionally, the IC50 value of betanin (19.2 μM) was lower than that of ascorbate (79.6 μM). The presence of betanin (0.05-1.0 μM) also inhibited ONOO- (0.5 mM)-dependent DNA strand cleavage in a concentration-dependent manner. These results suggest that betalains can protect cells from nitrosative stress in addition to protecting them from oxidative stresses. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/64661 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 崎浜 靖子
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