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Development of compartment for studies on the growth of protein crystals in space

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Title: Development of compartment for studies on the growth of protein crystals in space
Authors: Yamazaki, T. Browse this author →KAKEN DB
Tsukamoto, K. Browse this author
Yoshizaki, I. Browse this author
Fukuyama, S. Browse this author
Miura, H. Browse this author
Shimaoka, T. Browse this author
Maki, T. Browse this author
Oshi, K. Browse this author
Kimura, Y. Browse this author
Issue Date: 15-Mar-2016
Publisher: American Institute of Physics (AIP)
Journal Title: Review of Scientific Instruments
Volume: 87
Issue: 3
Start Page: 033107
Publisher DOI: 10.1063/1.4942961
PMID: 27036758
Abstract: To clarify the growth mechanism of a protein crystal, it is essential to measure its growth rate with respect to the supersaturation. We developed a compartment (growth cell) for measuring the growth rate (<0.1 nm s(-1)) of the face of a protein crystal at a controlled supersaturation by interferometry over a period of half a year in space. The growth cell mainly consists of quartz glass, in which the growth solution and a seed crystal are enclosed by capillaries, the screw sample holder, and a helical insert. To avoid the destruction of the cell and the evaporation of the water from the solution inside the cell, we selected the materials for these components with care. The equipment was successfully used to examine the growth of a lysozyme crystal at a controlled supersaturation in space, where convection is negligible because of the microgravity environment, thereby advancing our understanding of the mechanism of protein crystal growth from solution. The technique used to develop the growth cell is useful not only for space experiments but also for kinetic studies of materials with very slow growth and dissolution rates (<10(-3) nm s(-1)). (C) 2016 AIP Publishing LLC.
Rights: This article may be downloaded for personal use only. Any other use requires prior permission of the author and AIP Publishing. The following article appeared in Review of Scientific Instruments 87, 033107 (2016); doi: 10.1063/1.4942961 and may be found at .
Type: article
Appears in Collections:低温科学研究所 (Institute of Low Temperature Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 山﨑 智也

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