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Transport and uptake effects of marine complex lipid liposomes in small intestinal epithelial cell models

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/64702

Title: Transport and uptake effects of marine complex lipid liposomes in small intestinal epithelial cell models
Authors: Du, Lei Browse this author
Yang, Yu-Hong Browse this author
Xu, Jie Browse this author
Wang, Yu-Ming Browse this author
Xue, Chang-Hu Browse this author
Kurihara, Hideyuki Browse this author →KAKEN DB
Takahashi, Koretaro Browse this author →KAKEN DB
Issue Date: 15-Mar-2016
Publisher: Royal Society of Chemistry
Journal Title: Food & function
Volume: 7
Issue: 4
Start Page: 1904
End Page: 1914
Publisher DOI: 10.1039/c6fo00066e
PMID: 27001385
Abstract: Nowadays, marine complex lipids, including starfish phospholipids (SFP) and cerebrosides (SFC) separated from Asterias amurensis as well as sea cucumber phospholipids (SCP) and cerebrosides (SCC) isolated from Cucumaria frondosa, have received much attention because of their potent biological activities. However, little information is known on the transport and uptake of these lipids in liposome forms in small intestinal cells. Therefore, this study was undertaken to investigate the effects of these complex lipid liposomes on transport and uptake in Caco-2 and M cell monolayer models. The results revealed that SFP and SCP contained 42% and 47.9% eicosapentaenoic acid (EPA), respectively. The average particle sizes of liposomes prepared in this study were from 169 to 189 nm. We found that the transport of the liposomes across the M cell monolayer model was much higher than the Caco-2 cell monolayer model. The liposomes consisting of SFP or SCP showed significantly higher transport and uptake than soy phospholipid (soy-PL) liposomes in both Caco-2 and M cell monolayer models. Our results also exhibited that treatment with 1 mM liposomes composed of SFP or SCP for 3 h tended to increase the EPA content in phospholipid fractions of both differentiated Caco-2 and M cells. Moreover, it was also found that the hybrid liposomes consisting of SFP/SFC/cholesterol (Chol) revealed higher transport and uptake across the M cell monolayer in comparison with other liposomes. Furthermore, treatment with SFP/SFC/Chol liposomes could notably decrease the trans-epithelial electrical resistance (TEER) values of Caco-2 and M cell monolayers. The present data also showed that the cell viability of differentiated Caco-2 and M cells was not affected after the treatment with marine complex lipids or soy-PL liposomes. Based on the data in this study, it was suggested that marine complex lipid liposomes exhibit prominent transport and uptake in small intestinal epithelial cell models.
Type: article (author version)
URI: http://hdl.handle.net/2115/64702
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高橋 是太郎

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