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An optimistic protein assembly from sequence reads salvaged an uncharacterized segment of mouse picobirnavirus
This item is licensed under:Creative Commons Attribution 4.0 International
| Title: | An optimistic protein assembly from sequence reads salvaged an uncharacterized segment of mouse picobirnavirus |
| Authors: | Gonzalez, Gabriel Browse this author | | Sasaki, Michihito Browse this author →KAKEN DB | | Burkitt-Gray, Lucy Browse this author | | Kamiya, Tomonori Browse this author | | Tsuji, Noriko M. Browse this author | | Sawa, Hirofumi Browse this author →KAKEN DB | | Ito, Kimihito Browse this author →KAKEN DB |
| Issue Date: | 10-Jan-2017 |
| Publisher: | Nature Publishing Group |
| Journal Title: | Scientific Reports |
| Volume: | 7 |
| Start Page: | 40447 |
| Publisher DOI: | 10.1038/srep40447 |
| Abstract: | Advances in Next Generation Sequencing technologies have enabled the generation of millions of sequences from microorganisms. However, distinguishing the sequence of a novel species from sequencing errors remains a technical challenge when the novel species is highly divergent from the closest known species. To solve such a problem, we developed a new method called Optimistic Protein Assembly from Reads (OPAR). This method is based on the assumption that protein sequences could be more conserved than the nucleotide sequences encoding them. By taking advantage of metagenomics, bioinformatics and conventional Sanger sequencing, our method successfully identified all coding regions of the mouse picobirnavirus for the first time. The salvaged sequences indicated that segment 1 of this virus was more divergent from its homologues in other Picobirnaviridae species than segment 2. For this reason, only segment 2 of mouse picobirnavirus has been detected in previous studies. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/64775 |
| Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 伊藤 公人
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