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Sclerostin enhances adipocyte differentiation in 3T3-L1 cells
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| Title: | Sclerostin enhances adipocyte differentiation in 3T3-L1 cells |
| Other Titles: | Sclerostin は 3T3-L1 細胞における脂肪細胞分化を促進する |
| Authors: | 浮田, 万由美 Browse this author |
| Issue Date: | 24-Mar-2016 |
| Publisher: | Hokkaido University |
| Abstract: | Sclerostin, a secreted protein encoded by the Sost gene, is produced byosteocytes and is inhibited by osteoblast differentiation and bone formation. Recently, afunctional association between bone and fat tissue has been suggested, and acorrelation between circulating sclerostin levels and lipid metabolism has been reportedin humans. However, the effects of sclerostin on adipogenesis remain unexplored. Inthe present study, we examined the role of sclerostin in regulating adipocytedifferentiation using 3T3-L1 preadipocytes. In these cells, sclerostin enhancedadipocyte-specific gene expression and the accumulation of lipid deposits. Sclerostinalso upregulated CCAAT/enhancer binding protein β expression but not cellproliferation and caspase-3/7 activities. Sclerostin also attenuated canonicalWnt3a-inhibited adipocyte differentiation. Recently, the transcriptional modulator TAZhas been involved in the canonical Wnt signaling pathway. Sclerostin reducedTAZ-responsive transcriptional activity and TAZ-responsive gene expression.Transfection of 3T3-L1 cells with TAZ siRNA increased the lipid deposits andadipogenic gene expression. These results show that sclerostin upregulates adipocytedifferentiation in 3T3-L1 cells, suggesting a possible role for the osteocyte-derivedsclerostin as a regulator of fat metabolism and as a reciprocal regulator of bone andadipose tissues metabolism. |
| Conffering University: | 北海道大学 |
| Degree Report Number: | 甲第12141号 |
| Degree Level: | 博士 |
| Degree Discipline: | 歯学 |
| Examination Committee Members: | (主査) 教授 山口 泰彦, 教授 田村 正人, 教授 網塚 憲生 |
| Degree Affiliation: | 歯学研究科（口腔医学専攻） |
| Type: | theses (doctoral) |
| URI: | http://hdl.handle.net/2115/64811 |
| Appears in Collections: | 学位論文 (Theses) > 博士 （歯学）
課程博士 (Doctorate by way of Advanced Course) > 歯学院(Graduate School of Dental Medicine)
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