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Heat treatment of curdlan enhances the enzymatic production of biologically active beta-(1,3)-glucan oligosaccharides

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Title: Heat treatment of curdlan enhances the enzymatic production of biologically active beta-(1,3)-glucan oligosaccharides
Authors: Kumagai, Yuya Browse this author
Okuyama, Masayuki Browse this author →KAKEN DB
Kimura, Atsuo Browse this author →KAKEN DB
Keywords: GH64
beta-(1,3)-glucan oligosaccharides
Issue Date: 2-Aug-2016
Publisher: Elsevier
Journal Title: Carbohydrate Polymers
Volume: 146
Start Page: 396
End Page: 401
Publisher DOI: 10.1016/j.carbpol.2016.03.066
PMID: 27112889
Abstract: Biologically active beta-(1,3)-glucan oligosaccharides were prepared from curdlan using GH64 enzyme (KfGH64). KfGH64 showed low activity toward native curdlan; thereby pretreatment conditions of curdlan were evaluated. KfGH64 showed the highest activity toward curdlan with heat treatment. The most efficient pretreatment (90 degrees C for 0.5 h) converted approximately 60% of curdlan into soluble saccharides under the optimized enzyme reaction conditions (pH 5.5, 37 degrees C, 100 rpm mixing speed, 24 h, and 10 mu g of KfGH64/1 g of curdlan). The resulting products were predominantly laminaripentaose and a small amount of p-(1,3)-glucans with an average degree of polymerization (DP) of 13 and 130. The products did not contain small oligosaccharides (DP< 5), indicating that the hydrolysis of heat-treated curdlan by KfGH64 is a suitable method for the production of biologically active beta-(1,3)-glucan oligosaccharides. (C) 2016 Elsevier Ltd. All rights reserved.
Rights: ©2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木村 淳夫

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