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Heat treatment of curdlan enhances the enzymatic production of biologically active beta-(1,3)-glucan oligosaccharides
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| Title: | Heat treatment of curdlan enhances the enzymatic production of biologically active beta-(1,3)-glucan oligosaccharides |
| Authors: | Kumagai, Yuya Browse this author | | Okuyama, Masayuki Browse this author →KAKEN DB | | Kimura, Atsuo Browse this author →KAKEN DB |
| Keywords: | GH64 | | beta-(1,3)-glucan oligosaccharides | | Curdlan |
| Issue Date: | 2-Aug-2016 |
| Publisher: | Elsevier |
| Journal Title: | Carbohydrate Polymers |
| Volume: | 146 |
| Start Page: | 396 |
| End Page: | 401 |
| Publisher DOI: | 10.1016/j.carbpol.2016.03.066 |
| PMID: | 27112889 |
| Abstract: | Biologically active beta-(1,3)-glucan oligosaccharides were prepared from curdlan using GH64 enzyme (KfGH64). KfGH64 showed low activity toward native curdlan; thereby pretreatment conditions of curdlan were evaluated. KfGH64 showed the highest activity toward curdlan with heat treatment. The most efficient pretreatment (90 degrees C for 0.5 h) converted approximately 60% of curdlan into soluble saccharides under the optimized enzyme reaction conditions (pH 5.5, 37 degrees C, 100 rpm mixing speed, 24 h, and 10 mu g of KfGH64/1 g of curdlan). The resulting products were predominantly laminaripentaose and a small amount of p-(1,3)-glucans with an average degree of polymerization (DP) of 13 and 130. The products did not contain small oligosaccharides (DP< 5), indicating that the hydrolysis of heat-treated curdlan by KfGH64 is a suitable method for the production of biologically active beta-(1,3)-glucan oligosaccharides. (C) 2016 Elsevier Ltd. All rights reserved. |
| Rights: | ©2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/64878 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木村 淳夫
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