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Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure
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| Title: | Surface plasmon resonance for detecting clenbuterol: Influence of monolayer structure |
| Authors: | Suherman Browse this author | | Morita, Kinichi Browse this author | | Kawaguchi, Toshikazu Browse this author →KAKEN DB |
| Keywords: | Surface plasmon resonance | | Self-assembled monolayer | | Clenbuterol detection | | Indirect competitive inhibition immunoassay |
| Issue Date: | 30-Mar-2015 |
| Publisher: | Elsevier |
| Journal Title: | Applied Surface Science |
| Volume: | 332 |
| Start Page: | 229 |
| End Page: | 236 |
| Publisher DOI: | 10.1016/j.apsusc.2015.01.169 |
| Abstract: | Surface plasmon resonance sensor equipped with a fabricated immunosensor chip is used for detecting clenbuterol in this study. Since clenbuterol is a small analyte, indirect competitive inhibition immunoassay is employed. For fabricating the immunosurface, the Au-chip was functionalized by succinimidyl-terminated alkanethiol, and the terminal N-hydroxysuccinimide group of the self-assembled monolayer was either replaced with clenbuterol or blocked with ethanolamine. Scanning tunneling microscope experiments and electrochemical measurements depicted the domain structures of the succinimide group of succinimidyl-terminated propanethiol monolayer. The surface concentration and the orientation of succinimide group was significantly dependent on the concentration of dithiobis(succinimidyl) propionate (DSP) used in fabricating the monolayer. Furthermore, the structure of monolayer significantly influenced both the surface concentration and the orientation of clenbuterol on the sensor surface. Consequently, high coverage and standing-up configuration of clenbuterol showed high affinity for clenbuterol antibody. However, high affinity constant exhibited by the sensor surface was coupled with a low sensitivity. By contrast, lowest concentration of DSP solution (0.1 mM) used in fabricating the immunosurface showed a detection sensitivity of 3 ppt the highest reported sensitivity for clenbuterol. For regeneration the immunosurface, 0.1 M NaOH was used and the same sensor surface could be reused for performing >100 rapid immunoreaction. (C) 2015 Elsevier B.V. All rights reserved. |
| Rights: | © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/64909 |
| Appears in Collections: | 環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 川口 俊一
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