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Identification of molecular variants of the nonrestoring restorer-of-fertility 1 allele in sugar beet (Beta vulgaris L.)

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Title: Identification of molecular variants of the nonrestoring restorer-of-fertility 1 allele in sugar beet (Beta vulgaris L.)
Authors: Ohgami, Takashi Browse this author
Uchiyama, Daisuke Browse this author
Ue, Sachiyo Browse this author
Rika, Yui-Kurino Browse this author
Yoshida, Yu Browse this author
Kamei, Yoko Browse this author
Kuroda, Yosuke Browse this author
Taguchi, Kazunori Browse this author
Kubo, Tomohiko Browse this author →KAKEN DB
Keywords: cytoplasmic male sterility
F1 hybrid
genetic resources
marker-assisted selection
nuclear-mitochondrial interaction
Rf gene
Issue Date: Apr-2016
Publisher: Springer
Journal Title: Theoretical and Applied Genetics
Volume: 129
Issue: 4
Start Page: 675
End Page: 688
Publisher DOI: 10.1007/s00122-015-2656-0
PMID: 26714697
Abstract: Cytoplasmic male sterility is widely used for hybrid breeding of sugar beets. Specific genotypes with a nonsterility-inducing cytoplasm and a nonrestoring allele of restorer-of-fertility gene (rf) are called maintainers. The infrequent occurrence of the maintainer genotype evokes the need to diagnose rf alleles. Molecular analysis of Rf1, one of the sugar beet Rfs, revealed a high level of nucleotide sequence diversity, but three variants were tightly associated with maintainer selection in Japan. The question was raised whether this small number of variants would be seen in cases where a wider range of genetic resources was used for maintainer selection. Fifty-seven accessions registered as maintainers in the USDA germplasm collection were characterized in this study. Mitochondrial DNA types (mitotypes) of 551 plants were diagnosed based on minisatellite polymorphism. A mitotype associated with sterility-inducing (S) cytoplasm was identified in 58 plants, indicating S-cytoplasm contamination. The organization of rf1 was investigated by two PCR markers and DNA gel blot analysis. Eight haplotypes were found among the US maintainers, but subsequently two haplotypes were judged as restoring alleles after a test cross and another haplotype was not inherited by the progeny. Nucleotide sequences of rf1 regions in the remaining five haplotypes were compared, and despite the sequence diversity of the gene-flanking regions, the gene-coding regions were identified to be three types. Therefore, there are three rf1 variants in US maintainers, the same number as in the Japanese sugar beet germplasm collection. The implications of having a small repertoire of rf1 variants are discussed.
Rights: The final publication is available at
Type: article (author version)
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 久保 友彦

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