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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >
RANKL regulates differentiation of microfold cells in mouse nasopharynx-associated lymphoid tissue (NALT)
| Title: | RANKL regulates differentiation of microfold cells in mouse nasopharynx-associated lymphoid tissue (NALT) |
| Authors: | Mutoh, Mami Browse this author | | Kimura, Shunsuke Browse this author →KAKEN DB | | Takahashi-Iwanaga, Hiromi Browse this author →KAKEN DB | | Hisamoto, Meri Browse this author | | Iwanaga, Toshihiko Browse this author →KAKEN DB | | Iida, Junichiro Browse this author →KAKEN DB |
| Keywords: | Microfold cells (M cells) | | Follicle-associated epithelium | | Nasopharynx-associated lymphoid tissue (NALT) | | Glycoprotein 2 (GP2) | | Receptor activator of nuclear factor kappa-B ligand (RANKL) |
| Issue Date: | Apr-2016 |
| Publisher: | Springer |
| Journal Title: | Cell and Tissue Research |
| Volume: | 364 |
| Issue: | 1 |
| Start Page: | 175 |
| End Page: | 184 |
| Publisher DOI: | 10.1007/s00441-015-2309-2 |
| PMID: | 26553655 |
| Abstract: | Murine nasopharynx-associated lymphoid tissue (NALT), located at the base of the nasal cavity, serves as a major site for the induction of mucosal immune responses against airway antigens. The follicle-associated epithelium (FAE) covering the luminal surface of NALT is characterized by the presence of microfold cells (M cells), which take up and transport luminal antigens to lymphocytes. Glycoprotein 2 (GP2) has recently been identified as a reliable marker for M cells in Peyer's patches of the intestine. However, the expression of GP2 and other functional molecules in the M cells of NALT has not yet been examined. We have immunohistochemically detected GP2-expressing cells in the FAE of NALT and the simultaneous expression of other intestinal M-cell markers, namely Tnfaip2, CCL9, and Spi-B. These cells have been further identified as M cells because of their higher uptake capacity of luminal microbeads. Electron microscopic observations have shown that GP2-expressing cells on the FAE display morphological features typical of M cells: they possess short microvilli and microfolds on the luminal surface and are closely associated with intraepithelial lymphocytes. We have also found that the receptor activator of nuclear factor kappa-B ligand (RANKL) is expressed by stromal cells underneath the FAE, which provides its receptor RANK. The administration of RANKL markedly increases the number of GP2+Tnfaip2+ cells on the NALT FAE and that of intestinal M cells. These results suggest that GP2+Tnfaip2+ cells in NALT are equivalent to intestinal M cells, and that RANKL-RANK signaling induces their differentiation. |
| Rights: | The final publication is available at link.springer.com |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/64947 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 木村 俊介
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