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Environmental DNA metabarcoding reveals local fish communities in a species-rich coastal sea
This item is licensed under:Creative Commons Attribution 4.0 International
| Title: | Environmental DNA metabarcoding reveals local fish communities in a species-rich coastal sea |
| Authors: | Yamamoto, Satoshi Browse this author | | Masuda, Reiji Browse this author | | Sato, Yukuto Browse this author | | Sado, Tetsuya Browse this author | | Araki, Hitoshi Browse this author →KAKEN DB | | Kondoh, Michio Browse this author | | Minamoto, Toshifumi Browse this author | | Miya, Masaki Browse this author |
| Issue Date: | 12-Jan-2017 |
| Publisher: | Nature Publishing |
| Journal Title: | Scientific Reports |
| Volume: | 7 |
| Start Page: | 40368 |
| Publisher DOI: | 10.1038/srep40368 |
| Abstract: | Environmental DNA (eDNA) metabarcoding has emerged as a potentially powerful tool to assess aquatic community structures. However, the method has hitherto lacked field tests that evaluate its effectiveness and practical properties as a biodiversity monitoring tool. Here, we evaluated the ability of eDNA metabarcoding to reveal fish community structures in species-rich coastal waters. Highperformance fish-universal primers and systematic spatial water sampling at 47 stations covering ~11 km2 revealed the fish community structure at a species resolution. The eDNA metabarcoding based on a 6-h collection of water samples detected 128 fish species, of which 62.5% (40 species) were also observed by underwater visual censuses conducted over a 14-year period. This method also detected other local fishes (≥23 species) that were not observed by the visual censuses. These eDNA metabarcoding features will enhance marine ecosystem-related research, and the method will potentially become a standard tool for surveying fish communities. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/65121 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 荒木 仁志
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