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Environmental DNA metabarcoding reveals local fish communities in a species-rich coastal sea

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Title: Environmental DNA metabarcoding reveals local fish communities in a species-rich coastal sea
Authors: Yamamoto, Satoshi Browse this author
Masuda, Reiji Browse this author
Sato, Yukuto Browse this author
Sado, Tetsuya Browse this author
Araki, Hitoshi Browse this author →KAKEN DB
Kondoh, Michio Browse this author
Minamoto, Toshifumi Browse this author
Miya, Masaki Browse this author
Issue Date: 12-Jan-2017
Publisher: Nature Publishing
Journal Title: Scientific Reports
Volume: 7
Start Page: 40368
Publisher DOI: 10.1038/srep40368
Abstract: Environmental DNA (eDNA) metabarcoding has emerged as a potentially powerful tool to assess aquatic community structures. However, the method has hitherto lacked field tests that evaluate its effectiveness and practical properties as a biodiversity monitoring tool. Here, we evaluated the ability of eDNA metabarcoding to reveal fish community structures in species-rich coastal waters. Highperformance fish-universal primers and systematic spatial water sampling at 47 stations covering ~11 km2 revealed the fish community structure at a species resolution. The eDNA metabarcoding based on a 6-h collection of water samples detected 128 fish species, of which 62.5% (40 species) were also observed by underwater visual censuses conducted over a 14-year period. This method also detected other local fishes (≥23 species) that were not observed by the visual censuses. These eDNA metabarcoding features will enhance marine ecosystem-related research, and the method will potentially become a standard tool for surveying fish communities.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒木 仁志

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