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Effect of Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Rats With Acute and Chronic Pancreatitis

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タイトル: Effect of Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Rats With Acute and Chronic Pancreatitis
その他のタイトル: Fetal membrane-derived MSC for pancreatitis
著者: Kawakubo, Kazumichi 著作を一覧する
Ohnishi, Shunsuke 著作を一覧する
Fujita, Hirotoshi 著作を一覧する
Kuwatani, Masaki 著作を一覧する
Onishi, Reizo 著作を一覧する
Masamune, Atsushi 著作を一覧する
Takeda, Hiroshi 著作を一覧する
Sakamoto, Naoya 著作を一覧する
キーワード: mesenchymal stem cells
acute pancreatitis
chronic pancreatitis
fetal membrane
発行日: 2016年 5月
出版者: Wolters Kluwer Health
誌名: Pancreas : Journal of Neuroendocrine Tumors and Pancreatic Diseases and Sciences
巻: 45
号: 5
開始ページ: 707
終了ページ: 713
出版社 DOI: 10.1097/MPA.0000000000000541
PMID: 26646279
抄録: Objectives: Mesenchymal stem cells (MSCs) are a valuable cell source in regenerative medicine and can be isolated from fetal membranes (FMs), particularly amniotic membranes. We investigated the effect of rat FM-derived MSCs (rFM-MSCs) and human amnion-derived MSCs (hAMSCs) on the inflammatory reaction in vitro and therapeutic effects in rats with acute and chronic pancreatitis. Methods: Effect of rFM-MSCs or hAMSC-conditioned medium was investigated in vitro. Acute pancreatitis was induced by intraductal injection of 4% taurocholate, and rFM-MSCs were transplanted intravenously. Chronic pancreatitis was induced by intravenous injection of 5 mg/kg dibutyltin dichloride, and hAMSCs were transplanted intravenously. Results: The inflammatory reaction of macrophages induced by lipopolysaccharide and trypsin was significantly suppressed by rFM-MSC co-culture. Pancreatic acinar cell injury induced by cerulein was significantly ameliorated by hAMSC-conditioned medium. Pancreatic stellate cell activation induced by TNF-α was significantly decreased by hAMSC-conditioned medium. Transplantation of rFM-MSCs significantly reduced the histological score and infiltration of CD68-positive macrophages in the rat pancreas. hAMSC transplantation significantly decreased the expression of MCP-1 and attenuated the downregulation of amylase expression in the pancreas. Conclusions: Transplantation of FM-MSCs and AMSCs suppressed the inflammatory reaction of acute and chronic pancreatitis in rats.
Rights: This is a non-final version of an article published in final form in Pancreas, Volume 45, Issue 5, 2016, pp.707–713.
Relation (URI):
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 大西 俊介


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