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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Medicine / Faculty of Medicine >
Peer-reviewed Journal Articles, etc >
Effect of Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Rats With Acute and Chronic Pancreatitis
| Title: | Effect of Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Rats With Acute and Chronic Pancreatitis |
| Other Titles: | Fetal membrane-derived MSC for pancreatitis |
| Authors: | Kawakubo, Kazumichi Browse this author | | Ohnishi, Shunsuke Browse this author →KAKEN DB | | Fujita, Hirotoshi Browse this author | | Kuwatani, Masaki Browse this author →KAKEN DB | | Onishi, Reizo Browse this author | | Masamune, Atsushi Browse this author →KAKEN DB | | Takeda, Hiroshi Browse this author →KAKEN DB | | Sakamoto, Naoya Browse this author →KAKEN DB |
| Keywords: | mesenchymal stem cells | | acute pancreatitis | | chronic pancreatitis | | macrophages | | fetal membrane | | amnion |
| Issue Date: | May-2016 |
| Publisher: | Wolters Kluwer Health |
| Journal Title: | Pancreas : Journal of Neuroendocrine Tumors and Pancreatic Diseases and Sciences |
| Volume: | 45 |
| Issue: | 5 |
| Start Page: | 707 |
| End Page: | 713 |
| Publisher DOI: | 10.1097/MPA.0000000000000541 |
| PMID: | 26646279 |
| Abstract: | Objectives: Mesenchymal stem cells (MSCs) are a valuable cell source in regenerative medicine and can be isolated from fetal membranes (FMs), particularly amniotic membranes. We investigated the effect of rat FM-derived MSCs (rFM-MSCs) and human amnion-derived MSCs (hAMSCs) on the inflammatory reaction in vitro and therapeutic effects in rats with acute and chronic pancreatitis. Methods: Effect of rFM-MSCs or hAMSC-conditioned medium was investigated in vitro. Acute pancreatitis was induced by intraductal injection of 4% taurocholate, and rFM-MSCs were transplanted intravenously. Chronic pancreatitis was induced by intravenous injection of 5 mg/kg dibutyltin dichloride, and hAMSCs were transplanted intravenously. Results: The inflammatory reaction of macrophages induced by lipopolysaccharide and trypsin was significantly suppressed by rFM-MSC co-culture. Pancreatic acinar cell injury induced by cerulein was significantly ameliorated by hAMSC-conditioned medium. Pancreatic stellate cell activation induced by TNF-α was significantly decreased by hAMSC-conditioned medium. Transplantation of rFM-MSCs significantly reduced the histological score and infiltration of CD68-positive macrophages in the rat pancreas. hAMSC transplantation significantly decreased the expression of MCP-1 and attenuated the downregulation of amylase expression in the pancreas. Conclusions: Transplantation of FM-MSCs and AMSCs suppressed the inflammatory reaction of acute and chronic pancreatitis in rats. |
| Rights: | This is a non-final version of an article published in final form in Pancreas, Volume 45, Issue 5, 2016, pp.707–713. |
| Relation: | http://journals.lww.com/pancreasjournal/pages/default.aspx |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/65180 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 大西 俊介
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