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Effect of Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Rats With Acute and Chronic Pancreatitis

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Title: Effect of Fetal Membrane-Derived Mesenchymal Stem Cell Transplantation in Rats With Acute and Chronic Pancreatitis
Other Titles: Fetal membrane-derived MSC for pancreatitis
Authors: Kawakubo, Kazumichi Browse this author
Ohnishi, Shunsuke Browse this author →KAKEN DB
Fujita, Hirotoshi Browse this author
Kuwatani, Masaki Browse this author →KAKEN DB
Onishi, Reizo Browse this author
Masamune, Atsushi Browse this author →KAKEN DB
Takeda, Hiroshi Browse this author →KAKEN DB
Sakamoto, Naoya Browse this author →KAKEN DB
Keywords: mesenchymal stem cells
acute pancreatitis
chronic pancreatitis
fetal membrane
Issue Date: May-2016
Publisher: Wolters Kluwer Health
Journal Title: Pancreas : Journal of Neuroendocrine Tumors and Pancreatic Diseases and Sciences
Volume: 45
Issue: 5
Start Page: 707
End Page: 713
Publisher DOI: 10.1097/MPA.0000000000000541
PMID: 26646279
Abstract: Objectives: Mesenchymal stem cells (MSCs) are a valuable cell source in regenerative medicine and can be isolated from fetal membranes (FMs), particularly amniotic membranes. We investigated the effect of rat FM-derived MSCs (rFM-MSCs) and human amnion-derived MSCs (hAMSCs) on the inflammatory reaction in vitro and therapeutic effects in rats with acute and chronic pancreatitis. Methods: Effect of rFM-MSCs or hAMSC-conditioned medium was investigated in vitro. Acute pancreatitis was induced by intraductal injection of 4% taurocholate, and rFM-MSCs were transplanted intravenously. Chronic pancreatitis was induced by intravenous injection of 5 mg/kg dibutyltin dichloride, and hAMSCs were transplanted intravenously. Results: The inflammatory reaction of macrophages induced by lipopolysaccharide and trypsin was significantly suppressed by rFM-MSC co-culture. Pancreatic acinar cell injury induced by cerulein was significantly ameliorated by hAMSC-conditioned medium. Pancreatic stellate cell activation induced by TNF-α was significantly decreased by hAMSC-conditioned medium. Transplantation of rFM-MSCs significantly reduced the histological score and infiltration of CD68-positive macrophages in the rat pancreas. hAMSC transplantation significantly decreased the expression of MCP-1 and attenuated the downregulation of amylase expression in the pancreas. Conclusions: Transplantation of FM-MSCs and AMSCs suppressed the inflammatory reaction of acute and chronic pancreatitis in rats.
Rights: This is a non-final version of an article published in final form in Pancreas, Volume 45, Issue 5, 2016, pp.707–713.
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 大西 俊介

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