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Effect of alkanethiol molecular structure on sensitivity of surface plasmon resonance sensor

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Title: Effect of alkanethiol molecular structure on sensitivity of surface plasmon resonance sensor
Authors: Suherman Browse this author
Morita, Kinichi Browse this author
Kawaguchi, Toshikazu Browse this author →KAKEN DB
Keywords: Alkanethiol monolayer
Surface plasmon resonance
Indirect competitive inhibition
Issue Date: Apr-2015
Journal Title: Sensors and Actuators B: Chemical
Volume: 210
Start Page: 768
End Page: 775
Publisher DOI: 10.1016/j.snb.2015.01.026
Abstract: This work aims to find the key factor determining the detection limit for an indirect competitive inhibition immunoassay using surface plasmon resonance (SPR) sensing. In our previous work, the thiol solution concentration used in a self-assembly process highly affected the alkanethiol monolayer structure on the sensor surface [Suherman et al., 2014]. It was noticed that the monolayer structure determined the immunoassay sensitivity due to the orientation and the surface concentration of antigen in domain structure of monolayer. To study the effect of orientation of antigen, we examined here three types of alkanethiol compounds: dithiobis(succinimidyl undecanoate) (DSU; straight-chain alkanethiol), carboxy-EG6-undecanethiol (CEG6; flexible-chain alkanethiol), and 3,3´-dithiobis[N-(5-amino-5-carboxypentyl)propionamide-N,N´-diacetic acid] (C2-NTA; three-branched alkanethiol). By electrochemical reductive desorption, the surface concentration of DSU, CEG6, and C2-NTA were estimated to be 6.6 × 10–10, 8.5 × 10–10, 4.6 × 10–10 mol cm–2, respectively. Subsequently, SPR suggested that the ratios of immobilized antigen (clenbuterol) per thiol were 0.13, 0.16, and 0.15 for DSU, CEG6, and C2-NTA, respectively. This suggests that the amount of immobilized clenbuterol was defined by the molecular size of clenbuterol. Sensor surface structures on a molecular scale were evaluated using high-resolution scanning tunneling microscopy (STM), which showed tilted clenbuterol with DSU > CEG6 > C2-NTA. For SPR sensing of clenbuterol, C2-NTA showed the highest sensitivity (LOD = 10 ppt) among the examined alkanethiols, because clenbuterol formed a laid-on structure. Based on kinetics, the key factor for sensing performance is discussed.
Rights: © 2015. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
Type: article (author version)
Appears in Collections:環境科学院・地球環境科学研究院 (Graduate School of Environmental Science / Faculty of Environmental Earth Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川口 俊一

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