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Hokkaido University Collection of Scholarly and Academic Papers >
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Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid
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| Title: | Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid |
| Authors: | Havelka, Miloš Browse this author | | Fujimoto, Takafumi Browse this author | | Hagihara, Seishi Browse this author | | Adachi, Shinji Browse this author →KAKEN DB | | Arai, Katsutoshi Browse this author →KAKEN DB |
| Issue Date: | 10-May-2017 |
| Journal Title: | Scientific Reports |
| Volume: | 7 |
| Issue: | 1 |
| Start Page: | 1694 |
| Publisher DOI: | 10.1038/s41598-017-01768-3 |
| Abstract: | Sturgeons (Acipenseriformes) are among the most endangered species in the world due to fragmentation and destruction of their natural habitats and to overexploitation, mainly for highly priced caviar. This has led to the development of sturgeon culture, originally for reintroduction, but more recently for caviar production. In both cases, accurate species identification is essential. We report a new tool for accurate identification of Huso huso and Acipenser ruthenus based on nuclear DNA markers. We employed ddRAD sequencing to identify species-specific nucleotide variants, which served as specific binding sites for diagnostic primers. The primers allowed identification of Huso huso and Acipenser ruthenus as well as their discrimination from A. baerii, A. schrenckii, A. gueldenstaedtii, A. stellatus, A. persicus, A. mikadoi, A. transmontanus, and H. dauricus and identification of A. ruthenus and H. huso hybrids with these species, except hybrid between A. ruthenus and A. stellatus. The speciesspecific primers also allowed identification of bester (H. huso × A. ruthenus), the most commercially exploited sturgeon hybrid. The tool, based on simple PCR and gel electrophoresis, is rapid, inexpensive, and reproducible. It will contribute to conservation of remaining wild populations of A. ruthenus and H. huso, as well as to traceability of their products. |
| Rights: | https://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/65240 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 荒井 克俊
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