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Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/65240

Title: Nuclear DNA markers for identification of Beluga and Sterlet sturgeons and their interspecific Bester hybrid
Authors: Havelka, Miloš Browse this author
Fujimoto, Takafumi Browse this author
Hagihara, Seishi Browse this author
Adachi, Shinji Browse this author →KAKEN DB
Arai, Katsutoshi Browse this author →KAKEN DB
Issue Date: 10-May-2017
Journal Title: Scientific Reports
Volume: 7
Issue: 1
Start Page: 1694
Publisher DOI: 10.1038/s41598-017-01768-3
Abstract: Sturgeons (Acipenseriformes) are among the most endangered species in the world due to fragmentation and destruction of their natural habitats and to overexploitation, mainly for highly priced caviar. This has led to the development of sturgeon culture, originally for reintroduction, but more recently for caviar production. In both cases, accurate species identification is essential. We report a new tool for accurate identification of Huso huso and Acipenser ruthenus based on nuclear DNA markers. We employed ddRAD sequencing to identify species-specific nucleotide variants, which served as specific binding sites for diagnostic primers. The primers allowed identification of Huso huso and Acipenser ruthenus as well as their discrimination from A. baerii, A. schrenckii, A. gueldenstaedtii, A. stellatus, A. persicus, A. mikadoi, A. transmontanus, and H. dauricus and identification of A. ruthenus and H. huso hybrids with these species, except hybrid between A. ruthenus and A. stellatus. The speciesspecific primers also allowed identification of bester (H. huso × A. ruthenus), the most commercially exploited sturgeon hybrid. The tool, based on simple PCR and gel electrophoresis, is rapid, inexpensive, and reproducible. It will contribute to conservation of remaining wild populations of A. ruthenus and H. huso, as well as to traceability of their products.
Rights: https://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/65240
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 荒井 克俊

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