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A Powerful CRISPR/Cas9-Based Method for Targeted Transcriptional Activation

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Title: A Powerful CRISPR/Cas9-Based Method for Targeted Transcriptional Activation
Authors: Katayama, Shota Browse this author
Moriguchi, Tetsuo Browse this author →KAKEN DB
Ohtsu, Naoki Browse this author
Kondo, Toru Browse this author →KAKEN DB
Keywords: CRISPR/Cas9
epigenetics
gene expression
gene technology
synthetic biology
DNA methylation
mRNA
Issue Date: 23-May-2016
Publisher: Wiley
Journal Title: Angewandte chemie. international edition
Volume: 55
Issue: 22
Start Page: 6452
End Page: 6456
Publisher DOI: 10.1002/anie.201601708
PMID: 27079176
Abstract: Targeted transcriptional activation of endogenous genes is important for understanding physiological transcriptional networks, synthesizing genetic circuits, and inducing cellular phenotype changes. The CRISPR/Cas9 system has great potential to achieve this purpose, however, it has not yet been successfully used to efficiently activate endogenous genes and induce changes in cellular phenotype. A powerful method for transcriptional activation by using CRISPR/Cas9 was developed. Replacement of a methylated promoter with an unmethylated one by CRISPR/Cas9 was sufficient to activate the expression of the neural cell gene OLIG2 and the embryonic stem cell gene NANOG in HEK293T cells. Moreover, CRISPR/Cas9-based OLIG2 activation induced the embryonic carcinoma cell line NTERA-2 to express the neuronal marker βIII-tubulin.
Rights: This is the peer reviewed version of the following article: S. Katayama, T. Moriguchi, N. Ohtsu, T. Kondo, Angew. Chem. Int. Ed. 2016, 55, 6452, which has been published in final form at http://dx.doi.org/10.1002/anie.201601708. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
https://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/65578
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 近藤 亨

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