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A Powerful CRISPR/Cas9-Based Method for Targeted Transcriptional Activation
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Title: | A Powerful CRISPR/Cas9-Based Method for Targeted Transcriptional Activation |
Authors: | Katayama, Shota Browse this author | Moriguchi, Tetsuo Browse this author →KAKEN DB | Ohtsu, Naoki Browse this author | Kondo, Toru Browse this author →KAKEN DB |
Keywords: | CRISPR/Cas9 | epigenetics | gene expression | gene technology | synthetic biology | DNA methylation | mRNA |
Issue Date: | 23-May-2016 |
Publisher: | Wiley |
Journal Title: | Angewandte chemie. international edition |
Volume: | 55 |
Issue: | 22 |
Start Page: | 6452 |
End Page: | 6456 |
Publisher DOI: | 10.1002/anie.201601708 |
PMID: | 27079176 |
Abstract: | Targeted transcriptional activation of endogenous genes is important for understanding physiological transcriptional networks, synthesizing genetic circuits, and inducing cellular phenotype changes. The CRISPR/Cas9 system has great potential to achieve this purpose, however, it has not yet been successfully used to efficiently activate endogenous genes and induce changes in cellular phenotype. A powerful method for transcriptional activation by using CRISPR/Cas9 was developed. Replacement of a methylated promoter with an unmethylated one by CRISPR/Cas9 was sufficient to activate the expression of the neural cell gene OLIG2 and the embryonic stem cell gene NANOG in HEK293T cells. Moreover, CRISPR/Cas9-based OLIG2 activation induced the embryonic carcinoma cell line NTERA-2 to express the neuronal marker βIII-tubulin. |
Rights: | This is the peer reviewed version of the following article: S. Katayama, T. Moriguchi, N. Ohtsu, T. Kondo, Angew. Chem. Int. Ed. 2016, 55, 6452, which has been published in final form at http://dx.doi.org/10.1002/anie.201601708. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/65578 |
Appears in Collections: | 遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 近藤 亨
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