| Title: | Conserved Roles of Fibroblast Growth Factor Receptor 2 Signaling in the Regulation of Inner Cell Mass Development in Bovine Blastocysts |
| Authors: | Akizawa, Hiroki Browse this author |
| Nagatomo, Hiroaki Browse this author |
| Odagiri, Haruka Browse this author |
| Kohri, Nanami Browse this author |
| Yamauchi, Nobuhiko Browse this author →KAKEN DB |
| Yanagawa, Yojiro Browse this author |
| Nagano, Masashi Browse this author →KAKEN DB |
| Takahashi, Masashi Browse this author →KAKEN DB |
| Kawahara, Manabu Browse this author →KAKEN DB |
| Keywords: | fibroblast growth factor signaling |
| inner cell mass |
| bovine |
| blastocyst |
| cell-lineage specification |
| Issue Date: | Jun-2016 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | Molecular reproduction and development |
| Volume: | 83 |
| Issue: | 6 |
| Start Page: | 516 |
| End Page: | 525 |
| Publisher DOI: | 10.1002/mrd.22646 |
| PMID: | 27060901 |
| Abstract: | A common process during preimplantation mammalian development is blastocyst formation, which utilizes signaling through fibroblast growth factor receptor 2 (FGFR2), yet the mechanisms through which FGFR2 signaling affect preimplantation development in bovine embryos remain incompletely understood. Here, we used RNA-interference to investigate the in vitro development, the frequency of blastomere apoptosis, and the mRNA expression of developmental marker genes in FGF receptor 2-knockdown (FGFR2-KD) bovine embryos. A reduction in FGFR2 mRNA did not affect preimplantation development or the frequency of apoptotic blastomeres, but did enhanced proliferation of the inner cell mass in blastocysts (P< 0.05) -which differs from the phenotype reported for bovine embryos using a pharmacological approach (treatment with the pan-FGFR blocker PD173074), but agrees with previous results obtained using mouse embryos. Moreover, the expression of an epiblast marker gene, NANOG, and a primitive endoderm marker gene, GATA6, remained unchanged, whereas the expression of another primitive endoderm marker gene, HNF4A, was significantly reduced in FGFR2-KD embryos. Therefore, FGFR2 signaling appears to be associated with the regulation of inner cell mass development and proliferation during blastocyst formation in cattle. |
| Rights: | This is the peer reviewed version of the following article: Molecular reproduction and development;83(6), pp516-525, 2017, which has been published in final form at 10.1002/mrd.22646. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/65786 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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