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PSD-95 regulates synaptic kainate receptors at mouse hippocampal mossy fiber-CA3 synapses


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タイトル: PSD-95 regulates synaptic kainate receptors at mouse hippocampal mossy fiber-CA3 synapses
著者: Suzuki, Etsuko 著作を一覧する
Kamiya, Haruyuki 著作を一覧する
キーワード: Kainate receptor
Postsynaptic density
発行日: 2016年 6月
出版者: Elsevier
誌名: Neuroscience research
巻: 107
開始ページ: 14
終了ページ: 19
出版社 DOI: 10.1016/j.neures.2015.12.011
抄録: Kainate-type glutamate receptors (KARs) are the third class of ionotropic glutamate receptors whose activation leads to the unique roles in regulating synaptic transmission and circuit functions. In contrast to AMPA receptors (AMPARs), little is known about the mechanism of synaptic localization of KARs. PSD-95, a major scaffold protein of the postsynaptic density, is a candidate molecule that regulates the synaptic KARs. Although PSD-95 was shown to bind directly to KARs subunits, it has not been tested whether PSD-95 regulates synaptic KARs in intact synapses. Using PSD-95 knockout mice, we directly investigated the role of PSD-95 in the KARs-mediated components of synaptic transmission at hippocampal mossy fiber-CA3 synapse, one of the synapses with the highest density of KARs. Mossy fiber EPSCs consist of AMPA receptor (AMPAR)-mediated fast component and KAR-mediated slower component, and the ratio was significantly reduced in PSD-95 knockout mice. The size of KARs-mediated field EPSP reduced in comparison with the size of the fiber volley. Analysis of KARs-mediated miniature EPSCs also suggested reduced synaptic KARs. All the evidence supports critical roles of PSD-95 in regulating synaptic KARs.
Rights: © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license
資料タイプ: article (author version)
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 神谷 温之


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