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Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration : a Pilot Study

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Title: Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration : a Pilot Study
Other Titles: Fluorescence evaluation of EUS-FNA specimens
Authors: Kawakubo, Kazumichi Browse this author
Ohnishi, Shunsuke Browse this author →KAKEN DB
Hatanaka, Yutaka Browse this author →KAKEN DB
Hatanaka, Kanako C. Browse this author
Hosono, Hidetaka Browse this author
Kubota, Yoshimasa Browse this author
Kamiya, Mako Browse this author →KAKEN DB
Kuwatani, Masaki Browse this author →KAKEN DB
Kawakami, Hiroshi Browse this author →KAKEN DB
Urano, Yasuteru Browse this author
Sakamoto, Naoya Browse this author →KAKEN DB
Keywords: Endoscopic ultrasound-guided fine needle aspiration
Pancreatic tumor
Fluorescent imaging
Enzymatically activatable fluorescence probe
Rapid on-site evaluation
Issue Date: Jun-2016
Publisher: Springer
Journal Title: Molecular imaging and biology
Volume: 18
Issue: 3
Start Page: 463
End Page: 471
Publisher DOI: 10.1007/s11307-015-0898-5
PMID: 26431952
Abstract: Purpose: Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is the most reliable method for the histological diagnosis of pancreatic tumors. Rapid on-site fluorescence-guided histological diagnosis was evaluated by topically applying an enzymatically activatable probe onto the EUS-FNA samples; the probe fluoresces in the presence of γ-glutamyltranspeptidase (GGT). Procedures: We evaluated GGT expression in pancreatic cancer cell lines in vitro. EUS-FNA was performed in 10 pancreatic tumors. After topical application of the probe, signal intensity was measured using a fluorescence imaging system for 13 min. Results: GGT was expressed in Panc-1, AsPC-1, and AR42J, but not in KP4 cells. In samples from six cases, several regions of the specimens fluoresced and contained adequate tissue for pathological diagnosis. The remaining four non-fluorescent samples contained very small amounts of carcinoma, normal epithelial cells, or no epithelial cells. The signal intensity at 5 min was 25.5 ± 7.7 and 7.7 ± 0.5 in fluorescent and non-fluorescent regions, respectively (p < 0.05). Conclusions: Application of enzymatically activatable probe onto EUS-FNA samples would be feasible for the rapid evaluation of tissues suitable for histological diagnosis.
Rights: The final publication is available at Springer via http://dx.doi.org/10.1007/s11307-015-0898-5
Type: article (author version)
URI: http://hdl.handle.net/2115/65837
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 大西 俊介

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