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Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration : a Pilot Study

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この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/65837

タイトル: Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration : a Pilot Study
その他のタイトル: Fluorescence evaluation of EUS-FNA specimens
著者: Kawakubo, Kazumichi 著作を一覧する
Ohnishi, Shunsuke 著作を一覧する
Hatanaka, Yutaka 著作を一覧する
Hatanaka, Kanako C. 著作を一覧する
Hosono, Hidetaka 著作を一覧する
Kubota, Yoshimasa 著作を一覧する
Kamiya, Mako 著作を一覧する
Kuwatani, Masaki 著作を一覧する
Kawakami, Hiroshi 著作を一覧する
Urano, Yasuteru 著作を一覧する
Sakamoto, Naoya 著作を一覧する
キーワード: Endoscopic ultrasound-guided fine needle aspiration
Pancreatic tumor
Fluorescent imaging
Enzymatically activatable fluorescence probe
Rapid on-site evaluation
発行日: 2016年 6月
出版者: Springer
誌名: Molecular imaging and biology
巻: 18
号: 3
開始ページ: 463
終了ページ: 471
出版社 DOI: 10.1007/s11307-015-0898-5
抄録: Purpose: Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is the most reliable method for the histological diagnosis of pancreatic tumors. Rapid on-site fluorescence-guided histological diagnosis was evaluated by topically applying an enzymatically activatable probe onto the EUS-FNA samples; the probe fluoresces in the presence of γ-glutamyltranspeptidase (GGT). Procedures: We evaluated GGT expression in pancreatic cancer cell lines in vitro. EUS-FNA was performed in 10 pancreatic tumors. After topical application of the probe, signal intensity was measured using a fluorescence imaging system for 13 min. Results: GGT was expressed in Panc-1, AsPC-1, and AR42J, but not in KP4 cells. In samples from six cases, several regions of the specimens fluoresced and contained adequate tissue for pathological diagnosis. The remaining four non-fluorescent samples contained very small amounts of carcinoma, normal epithelial cells, or no epithelial cells. The signal intensity at 5 min was 25.5 ± 7.7 and 7.7 ± 0.5 in fluorescent and non-fluorescent regions, respectively (p < 0.05). Conclusions: Application of enzymatically activatable probe onto EUS-FNA samples would be feasible for the rapid evaluation of tissues suitable for histological diagnosis.
Rights: The final publication is available at Springer via http://dx.doi.org/10.1007/s11307-015-0898-5
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/65837
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 大西 俊介

 

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