| Title: | Feasibility of Using an Enzymatically Activatable Fluorescence Probe for the Rapid Evaluation of Pancreatic Tissue Obtained Using Endoscopic Ultrasound-Guided Fine Needle Aspiration : a Pilot Study |
| Other Titles: | Fluorescence evaluation of EUS-FNA specimens |
| Authors: | Kawakubo, Kazumichi Browse this author |
| Ohnishi, Shunsuke Browse this author →KAKEN DB |
| Hatanaka, Yutaka Browse this author →KAKEN DB |
| Hatanaka, Kanako C. Browse this author |
| Hosono, Hidetaka Browse this author |
| Kubota, Yoshimasa Browse this author |
| Kamiya, Mako Browse this author →KAKEN DB |
| Kuwatani, Masaki Browse this author →KAKEN DB |
| Kawakami, Hiroshi Browse this author →KAKEN DB |
| Urano, Yasuteru Browse this author |
| Sakamoto, Naoya Browse this author →KAKEN DB |
| Keywords: | Endoscopic ultrasound-guided fine needle aspiration |
| Pancreatic tumor |
| Fluorescent imaging |
| Enzymatically activatable fluorescence probe |
| Rapid on-site evaluation |
| Issue Date: | Jun-2016 |
| Publisher: | Springer |
| Journal Title: | Molecular imaging and biology |
| Volume: | 18 |
| Issue: | 3 |
| Start Page: | 463 |
| End Page: | 471 |
| Publisher DOI: | 10.1007/s11307-015-0898-5 |
| PMID: | 26431952 |
| Abstract: | Purpose: Endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) is the most reliable method for the histological diagnosis of pancreatic tumors. Rapid on-site fluorescence-guided histological diagnosis was evaluated by topically applying an enzymatically activatable probe onto the EUS-FNA samples; the probe fluoresces in the presence of γ-glutamyltranspeptidase (GGT). Procedures: We evaluated GGT expression in pancreatic cancer cell lines in vitro. EUS-FNA was performed in 10 pancreatic tumors. After topical application of the probe, signal intensity was measured using a fluorescence imaging system for 13 min. Results: GGT was expressed in Panc-1, AsPC-1, and AR42J, but not in KP4 cells. In samples from six cases, several regions of the specimens fluoresced and contained adequate tissue for pathological diagnosis. The remaining four non-fluorescent samples contained very small amounts of carcinoma, normal epithelial cells, or no epithelial cells. The signal intensity at 5 min was 25.5 ± 7.7 and 7.7 ± 0.5 in fluorescent and non-fluorescent regions, respectively (p < 0.05). Conclusions: Application of enzymatically activatable probe onto EUS-FNA samples would be feasible for the rapid evaluation of tissues suitable for histological diagnosis. |
| Rights: | The final publication is available at Springer via http://dx.doi.org/10.1007/s11307-015-0898-5 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/65837 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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