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Sclerostin Enhances Adipocyte Differentiation in 3T3-L1 Cells

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Title: Sclerostin Enhances Adipocyte Differentiation in 3T3-L1 Cells
Authors: Ukita, Mayumi Browse this author
Yamaguchi, Taihiko Browse this author →KAKEN DB
Ohata, Noboru Browse this author →KAKEN DB
Tamura, Masato Browse this author →KAKEN DB
Keywords: sclerostin
Issue Date: Jun-2016
Publisher: Wiley-Blackwell
Journal Title: Journal of cellular biochemistry
Volume: 117
Issue: 6
Start Page: 1419
End Page: 1428
Publisher DOI: 10.1002/jcb.25432
PMID: 26553151
Abstract: Sclerostin, a secreted protein encoded by the Sost gene, is produced by osteocytes and is inhibited by osteoblast differentiation and bone formation. Recently, a functional association between bone and fat tissue has been suggested, and a correlation between circulating sclerostin levels and lipid metabolism has been reported in humans. However, the effects of sclerostin on adipogenesis remain unexplored. In the present study, we examined the role of sclerostin in regulating adipocyte differentiation using 3T3-L1 preadipocytes. In these cells, sclerostin enhanced adipocyte-specific gene expression and the accumulation of lipid deposits. Sclerostin also upregulated CCAAT/enhancer binding protein expression but not cell proliferation and caspase-3/7 activities. Sclerostin also attenuated canonical Wnt3a-inhibited adipocyte differentiation. Recently, the transcriptional modulator TAZ has been involved in the canonical Wnt signaling pathway. Sclerostin reduced TAZ-responsive transcriptional activity and TAZ-responsive gene expression. Transfection of 3T3-L1 cells with TAZ siRNA increased the lipid deposits and adipogenic gene expression. These results show that sclerostin upregulates adipocyte differentiation in 3T3-L1 cells, suggesting a possible role for the osteocyte-derived sclerostin as a regulator of fat metabolism and as a reciprocal regulator of bone and adipose tissues metabolism. J. Cell. Biochem. 117: 1419-1428, 2016. (c) 2015 Wiley Periodicals, Inc.
Rights: This is the peer reviewed version of the following article: [Sclerostin enhances adipocyte differentiation in 3T3-L1 cells], which has been published in final form at []. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田村 正人

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