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Activation of inflammasomes in dendritic cells and macrophages byMycoplasma salivarium

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Title: Activation of inflammasomes in dendritic cells and macrophages byMycoplasma salivarium
Other Titles: Activation of inflammasomes by oral mycoplasma
Authors: Sugiyama, Masahiro Browse this author
Saeki, Ayumi Browse this author →KAKEN DB
Hasebe, Akira Browse this author →KAKEN DB
Kamesaki, Ryousuke Browse this author
Yoshida, Yasuhiro Browse this author →KAKEN DB
Kitagawa, Yoshimasa Browse this author →KAKEN DB
Suzuki, Toshihiko Browse this author
Shibata, Ken-ichiro Browse this author →KAKEN DB
Keywords: inflammasome
NLRP3
IL-1β
Mycoplasma salivarium
Mycoplasma pneumoniae
Issue Date: Jun-2016
Publisher: Wiley
Journal Title: Molecular Oral Microbiology
Volume: 31
Issue: 3
Start Page: 259
End Page: 269
Publisher DOI: 10.1111/omi.12117
PMID: 26177301
Abstract: Interleukin-1β (IL-1β) plays crucial roles in the pathogenesis of periodontal disease. IL-1β is produced after the processing of pro-IL-1β by caspase-1, which is activated by the inflammasome-a multiprotein complex comprising NLR, the adaptor protein ASC, and procaspase-1. Mycoplasma salivarium preferentially inhabits the gingival sulcus and the incidence and number of organisms in the oral cavity increase significantly with the progression of periodontal disease. To initially clarify the association of this organism with periodontal diseases, this study determined whether it induces IL-1β production by innate immune cells such as dendritic cells or macrophages by using Mycoplasma pneumoniae as a positive control. Both live and heat-killed M. salivarium and M. pneumoniae cells induced IL-1β production by XS106 murine dendritic cells as well as pyroptosis. The activities were significantly downregulated by silencing of caspase-1. Bone marrow-derived macrophage (BMMs) from wild-type and NLRP3-, ASC-, and caspase-1–deficient mice were examined for IL-1β production in response to these mycoplasmas. Live M. salivarium and M. pneumoniae cells almost completely lost the ability to induce IL-1β production by BMMs from ASC- and caspase-1– deficient mice. Their activities toward BMMs from NLRP3-deficient mice were significantly but not completely attenuated. These results suggest that live M. salivarium and M. pneumoniae cells can activate several types of inflammasomes including the NLRP3 inflammasome. M. salivarium as well as M. pneumoniae cells can activate THP-1 human monocytic cells to induce IL-1β production. Thus, the present finding that M. salivarium induces IL-1β production by dendritic cells and macrophages may suggest the association of this organism with periodontal diseases.
Rights: This is the peer reviewed version of the following article: [Activation of inflammasomes in dendritic cells and macrophages by Mycoplasma salivarium.], which has been published in final form at [http://doi.org/10.1111/omi.12117]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
URI: http://hdl.handle.net/2115/65843
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 柴田 健一郎

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