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Role of ATF5 in the invasive potential of diverse human cancer cell lines

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Title: Role of ATF5 in the invasive potential of diverse human cancer cell lines
Authors: Nukuda, Akihiro Browse this author
Endoh, Hiroki Browse this author
Yasuda, Motoaki Browse this author
Mizutani, Takeomi Browse this author
Kawabata, Kazushige Browse this author
Haga, Hisashi Browse this author →KAKEN DB
Keywords: Activating transcription factor 5
Integrin-alpha 2
Integrin-beta 1
Invasion
Spindle morphology
Issue Date: 3-Jun-2016
Publisher: Elsevier
Journal Title: Biochemical and biophysical research communications
Volume: 474
Issue: 3
Start Page: 509
End Page: 514
Publisher DOI: 10.1016/j.bbrc.2016.04.131
PMID: 27125458
Abstract: Activating transcription factor 5 (ATF5) is a member of the ATF/CAMP response element-binding protein family. Our research group recently revealed that ATF5 expression increases the invasiveness of human lung carcinoma cells. However, the effects of ATF5 on the invasive potential of other cancer cells lines remain unclear. Therefore, in this study, we investigated the role of ATF5 in the invasive activity of diverse human cancer cell lines. Invasiveness was assessed using Matrigel invasion assays. ATF5 knockdown resulted in decreased invasiveness in seven of eight cancer cell lines tested. These results suggest that ATF5 promotes invasiveness in several cancer cell lines. Furthermore, the roles of ATF5 in the invasiveness were evaluated in three-dimensional (3D) culture conditions. In 3D collagen gel, HT-1080 and MDA-MB-231 cells exhibited high invasiveness, with spindle morphology and high invasion speed. In both cell lines, knockdown of ATF5 resulted in rounded morphology and decreased invasion speed. Next, we showed that ATF5 induced integrin-alpha 2 and integrin-beta 1 expression and that the depletion of integrin-alpha 2 or integrin-beta 1 resulted in round morphology and decreased invasion speed. Our results suggest that ATF5 promotes invasion by inducing the expression of integrin-alpha 2 and integrin-beta 1 in several human cancer cell lines. (C) 2016 Elsevier Inc. All rights reserved.
Rights: © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
https://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/65863
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 芳賀 永

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