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Role of ATF5 in the invasive potential of diverse human cancer cell lines
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| Title: | Role of ATF5 in the invasive potential of diverse human cancer cell lines |
| Authors: | Nukuda, Akihiro Browse this author | | Endoh, Hiroki Browse this author | | Yasuda, Motoaki Browse this author | | Mizutani, Takeomi Browse this author | | Kawabata, Kazushige Browse this author | | Haga, Hisashi Browse this author →KAKEN DB |
| Keywords: | Activating transcription factor 5 | | Integrin-alpha 2 | | Integrin-beta 1 | | Invasion | | Spindle morphology |
| Issue Date: | 3-Jun-2016 |
| Publisher: | Elsevier |
| Journal Title: | Biochemical and biophysical research communications |
| Volume: | 474 |
| Issue: | 3 |
| Start Page: | 509 |
| End Page: | 514 |
| Publisher DOI: | 10.1016/j.bbrc.2016.04.131 |
| PMID: | 27125458 |
| Abstract: | Activating transcription factor 5 (ATF5) is a member of the ATF/CAMP response element-binding protein family. Our research group recently revealed that ATF5 expression increases the invasiveness of human lung carcinoma cells. However, the effects of ATF5 on the invasive potential of other cancer cells lines remain unclear. Therefore, in this study, we investigated the role of ATF5 in the invasive activity of diverse human cancer cell lines. Invasiveness was assessed using Matrigel invasion assays. ATF5 knockdown resulted in decreased invasiveness in seven of eight cancer cell lines tested. These results suggest that ATF5 promotes invasiveness in several cancer cell lines. Furthermore, the roles of ATF5 in the invasiveness were evaluated in three-dimensional (3D) culture conditions. In 3D collagen gel, HT-1080 and MDA-MB-231 cells exhibited high invasiveness, with spindle morphology and high invasion speed. In both cell lines, knockdown of ATF5 resulted in rounded morphology and decreased invasion speed. Next, we showed that ATF5 induced integrin-alpha 2 and integrin-beta 1 expression and that the depletion of integrin-alpha 2 or integrin-beta 1 resulted in round morphology and decreased invasion speed. Our results suggest that ATF5 promotes invasion by inducing the expression of integrin-alpha 2 and integrin-beta 1 in several human cancer cell lines. (C) 2016 Elsevier Inc. All rights reserved. |
| Rights: | © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/65863 |
| Appears in Collections: | 生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 芳賀 永
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