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Therapeutic effects of human amnion-derived mesenchymal stem cell transplantation and conditioned medium enema in rats with trinitrobenzene sulfonic acid-induced colitis

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タイトル: Therapeutic effects of human amnion-derived mesenchymal stem cell transplantation and conditioned medium enema in rats with trinitrobenzene sulfonic acid-induced colitis
著者: Miyamoto, Shuichi 著作を一覧する
Ohnishi, Shunsuke 著作を一覧する
Onishi, Reizo 著作を一覧する
Tsuchiya, Ikuki 著作を一覧する
Hosono, Hidetaka 著作を一覧する
Katsurada, Takehiko 著作を一覧する
Yamahara, Kenichi 著作を一覧する
Takeda, Hiroshi 著作を一覧する
Sakamoto, Naoya 著作を一覧する
キーワード: Mesenchymal stem cells
amnion
conditioned medium
trinitrobenzene sulfonic acid
colitis
発行日: 2017年
出版者: e-Century Publishing Corporation
誌名: American journal of translational research
巻: 9
号: 3
開始ページ: 940
終了ページ: 952
抄録: Cell therapy with mesenchymal stem cells (MSCs) is expected to provide a new strategy for the treatment of inflammatory bowel disease (IBD). Large amounts of MSCs can be obtained from human amnion. Therefore, we investigated the effect of transplantation of human amnion-derived MSCs (hAMSCs) or enema of conditioned medium (CM) from hAMSCs into rats with 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis. In the first experiment, 10-week-old male Sprague-Dawley rats were intravenously injected with hAMSCs (1 × 10^6 cells) 3 h after rectal administration of TNBS (45 mg/kg). In the second experiment, rats with TNBS-induced colitis received CM by enema into the colon for 3 days. Colitis was investigated by endoscopy, histology, immunohistochemistry, and by measuring mRNA expression of inflammatory mediators. Administration of hAMSCs or CM enema significantly improved the endoscopic score. In addition, these two interventions resulted in significantly decreased infiltration of neutrophils and monocytes/macrophages and decreased expression levels of TNF-α, CXCL1, and CCL2. In conclusion, transplantation of hAMSCs and CM enema provided significant improvement in rats with TNBS-induced colitis. CM from hAMSCs and hAMSCs may be new strategies for the treatment of IBD.
資料タイプ: article
URI: http://hdl.handle.net/2115/66142
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 大西 俊介

 

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