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17 beta-HSD Type 12-Like Is Responsible for MaturationInducing Hormone Synthesis During Oocyte Maturation in Masu Salmon

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Title: 17 beta-HSD Type 12-Like Is Responsible for MaturationInducing Hormone Synthesis During Oocyte Maturation in Masu Salmon
Authors: Ijiri, Shigeho Browse this author →KAKEN DB
Shibata, Yasushi Browse this author
Takezawa, Nonoha Browse this author
Kazeto, Yukinori Browse this author
Takatsuka, Naoki Browse this author
Kato, Erika Browse this author
Hagihara, Seishi Browse this author
Ozaki, Yuichi Browse this author
Adachi, Shinji Browse this author →KAKEN DB
Yamauchi, Kohei Browse this author
Nagahama, Yoshitaka Browse this author →KAKEN DB
Keywords: 17α,20β-dihydroxy-4-pregnen-3-one
20β-hydroxysteroid dehydrogenase
17β-hydroxysteroid dehydrogenase
Oocyte maturation
Granulosa, Masu salmon
Oncorhynchus masou
Issue Date: Mar-2017
Publisher: Endocrine Society
Journal Title: Endocrinology
Volume: 158
Issue: 3
Start Page: 627
End Page: 639
Publisher DOI: 10.1210/en.2016-1349
PMID: 27967235
Abstract: The maturation-inducing hormone 17(alpha), 20(beta)-dihydroxy-4-pregnen-3-one (DHP) was first identified in the amago salmon. Although carbonyl reductase-like 20 beta-hydroxysteroid dehydrogenase (CR/20b-HSD) was reported to convert 17 alpha-hydroxyprogesterone (17OHP) to DHP in rainbow trout, we previously found that CR/20 beta-HSD messenger RNA (mRNA) was not upregulated in stimulated granulosa cells from masu salmon, which suggested that DHP is synthesized by a different enzyme. Accordingly, the current study aimed to identify the specific 20 beta-hydroxysteroid dehydrogenase (20 beta-HSD) responsible for DHP production by granulosa cells during final oocyte maturation in masu salmon. RNA sequencing was performed on granulosa layers that were isolated from ovarian follicles at 1 month before ovulation and incubated with or without forskolin, which was used to mimic luteinizing hormone, and similar to 12 million reads were obtained, which yielded 71,062 contigs of > 100 bp. tBlastx analysis identified 1 contig (#f103496)as similar to 17 beta-hydroxysteroid dehydrogenase type 12 (hsd17 beta 12); however, because the full-length #f103496 sequence was different from hsd17 beta 12, it was termed hsd17 beta 12-like (hsd17 beta 12l). We found that mammalian cells transfected with full-length hsd171 beta 2l exhibited considerable 20b-HSD activity, as indicated by efficient conversion of exogenous 17OHP to DHP. In addition, we found that hsd17 beta 12l mRNA levels were consistently low in follicles during vitellogenic growth; however, the levels increased significantly during final oocytematuration. The levels of hsd17 beta 12lmRNA were also considerably increased in granulosa layers in which 20 beta-HSD activity was induced by salmon pituitary extract. Therefore, we suggest that hsd17 beta 12l, not CR/20 beta-HSD, is the 20 beta-HSD responsible for DHP production by granulosa cells in masu salmon during final oocytematuration.
Type: article (author version)
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 井尻 成保

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