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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
17 beta-HSD Type 12-Like Is Responsible for MaturationInducing Hormone Synthesis During Oocyte Maturation in Masu Salmon
| Title: | 17 beta-HSD Type 12-Like Is Responsible for MaturationInducing Hormone Synthesis During Oocyte Maturation in Masu Salmon |
| Authors: | Ijiri, Shigeho Browse this author →KAKEN DB | | Shibata, Yasushi Browse this author | | Takezawa, Nonoha Browse this author | | Kazeto, Yukinori Browse this author | | Takatsuka, Naoki Browse this author | | Kato, Erika Browse this author | | Hagihara, Seishi Browse this author | | Ozaki, Yuichi Browse this author | | Adachi, Shinji Browse this author →KAKEN DB | | Yamauchi, Kohei Browse this author | | Nagahama, Yoshitaka Browse this author →KAKEN DB |
| Keywords: | 17α,20β-dihydroxy-4-pregnen-3-one | | 20β-hydroxysteroid dehydrogenase | | 17β-hydroxysteroid dehydrogenase | | Oocyte maturation | | Granulosa, Masu salmon | | Oncorhynchus masou |
| Issue Date: | Mar-2017 |
| Publisher: | Endocrine Society |
| Journal Title: | Endocrinology |
| Volume: | 158 |
| Issue: | 3 |
| Start Page: | 627 |
| End Page: | 639 |
| Publisher DOI: | 10.1210/en.2016-1349 |
| PMID: | 27967235 |
| Abstract: | The maturation-inducing hormone 17(alpha), 20(beta)-dihydroxy-4-pregnen-3-one (DHP) was first identified in the amago salmon. Although carbonyl reductase-like 20 beta-hydroxysteroid dehydrogenase (CR/20b-HSD) was reported to convert 17 alpha-hydroxyprogesterone (17OHP) to DHP in rainbow trout, we previously found that CR/20 beta-HSD messenger RNA (mRNA) was not upregulated in stimulated granulosa cells from masu salmon, which suggested that DHP is synthesized by a different enzyme. Accordingly, the current study aimed to identify the specific 20 beta-hydroxysteroid dehydrogenase (20 beta-HSD) responsible for DHP production by granulosa cells during final oocyte maturation in masu salmon. RNA sequencing was performed on granulosa layers that were isolated from ovarian follicles at 1 month before ovulation and incubated with or without forskolin, which was used to mimic luteinizing hormone, and similar to 12 million reads were obtained, which yielded 71,062 contigs of > 100 bp. tBlastx analysis identified 1 contig (#f103496)as similar to 17 beta-hydroxysteroid dehydrogenase type 12 (hsd17 beta 12); however, because the full-length #f103496 sequence was different from hsd17 beta 12, it was termed hsd17 beta 12-like (hsd17 beta 12l). We found that mammalian cells transfected with full-length hsd171 beta 2l exhibited considerable 20b-HSD activity, as indicated by efficient conversion of exogenous 17OHP to DHP. In addition, we found that hsd17 beta 12l mRNA levels were consistently low in follicles during vitellogenic growth; however, the levels increased significantly during final oocytematuration. The levels of hsd17 beta 12lmRNA were also considerably increased in granulosa layers in which 20 beta-HSD activity was induced by salmon pituitary extract. Therefore, we suggest that hsd17 beta 12l, not CR/20 beta-HSD, is the 20 beta-HSD responsible for DHP production by granulosa cells in masu salmon during final oocytematuration. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/66278 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 井尻 成保
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