| Title: | Differential detection of cytoplasmic Wilms tumor 1 expression by immunohistochemistry, western blotting and mRNA quantification |
| Authors: | Maki, Takehiro Browse this author |
| Ikeda, Hiroaki Browse this author |
| Kuroda, Aki Browse this author |
| Kyogoku, Noriaki Browse this author |
| Yamamura, Yoshiyuki Browse this author |
| Tabata, Yukiko Browse this author |
| Abiko, Takehiro Browse this author |
| Tsuchikawa, Takahiro Browse this author →KAKEN DB |
| Hida, Yasuhiro Browse this author →KAKEN DB |
| Shichinohe, Toshiaki Browse this author →KAKEN DB |
| Tanaka, Eiichi Browse this author →KAKEN DB |
| Kaga, Kichizo Browse this author →KAKEN DB |
| Hatanaka, Kanako Browse this author |
| Matsuno, Yoshihiro Browse this author →KAKEN DB |
| Imai, Naoko Browse this author →KAKEN DB |
| Hirano, Satoshi Browse this author →KAKEN DB |
| Keywords: | immunoblotting |
| immunohistochemistry |
| neoplasms |
| reverse transcriptase polymerase chain reaction |
| Wilms Tumor 1 protein |
| Issue Date: | Jan-2017 |
| Publisher: | Spandidos Publications |
| Journal Title: | International journal of oncology |
| Volume: | 50 |
| Issue: | 1 |
| Start Page: | 129 |
| End Page: | 140 |
| Publisher DOI: | 10.3892/ijo.2016.3786 |
| PMID: | 27922671 |
| Abstract: | Wilms tumor 1 (WT1) is considered to be a promising target of cancer treatment because it has been reported to be frequently expressed at high levels in various malignancies. Although WT1-targeted cancer treatment has been initiated, conclusive detection methods for WT1 are not established. The present study aimed to consolidate immunohistochemistry for WT1 with statistical basis. Transfected cells with forced WT1 expression yielded specific western blot bands and nuclear immunostaining; cytoplasmic immunostaining was not specifically recognized. Immunohistochemistry, western blotting, and quantitative reverse transcriptase-polymerase chain reaction were performed in 35 human cell lines using multiple WT1 antibodies and their results were quantified. Relationships among the quantified results were statistically analyzed; the nuclear immunostaining positively correlated with western blot bands and mRNA expression levels, whereas cytoplasmic immunostaining did not. These results indicate that nuclear immunostaining reflects WT1 expression but cytoplasmic immunostaining does not. The nuclear immunostaining was barely (3/541) observed in primary cancer of esophagus, bile duct, pancreas and lung. Although the present study has some limitations, the results indicate that the cytoplasmic immunostaining does not correlate with actual WT1 expression and prompts researchers to carefully evaluate target molecule expression in treatment of cancer. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/66405 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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