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Isolation of Microsatellite Loci from the Onion Thrips,Thrips tabaci

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Title: Isolation of Microsatellite Loci from the Onion Thrips,Thrips tabaci
Authors: Kobayashi, Kazuya Browse this author
Hasegawa, Eisuke Browse this author →KAKEN DB
Keywords: genomic DNA
Menderian inheritance
reproductive mode
Issue Date: 18-Apr-2013
Publisher: University of Arizona Library
Journal Title: Journal of Insect Science
Volume: 13
Issue: 30
Start Page: 1
End Page: 5
Publisher DOI: 10.1673/031.013.3001
PMID: 23902377
Abstract: Thrips tabaci Lindeman (Thysanoptera: Thripidae), a cosmopolitan pest insect, is subdivided into at least three genetic lineages that have different biological characters, such as reproductive mode and resistibility to insecticides. Since the lineages are discriminated only by mitochondrial DNA, there is a possibility of gene flow among lineages at the genomic level. Nine polymorphic microsatellite loci were newly isolated from the taxon. Moderate to high levels of polymorphism were observed, with numbers of alleles ranging from 6 to 12 in 51 individuals. The mean observed and expected heterozygosities ranged from 0.1373 to 0.3725 and 0.5381 to 0.7748, respectively. Contrary to the expectation under Hardy-Weinberg’s equilibrium, six of the nine loci exhibited a reduction to homozygosities. However, we confirmed that alleles in all the loci were inherited as Mendeilan characteristics. These new loci will be useful to explore discrimination of lineages and population genetics in this species.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 長谷川 英祐

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