|
Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >
Isolation of Microsatellite Loci from the Onion Thrips,Thrips tabaci
This item is licensed under:Creative Commons Attribution 3.0 Unported
| Title: | Isolation of Microsatellite Loci from the Onion Thrips,Thrips tabaci |
| Authors: | Kobayashi, Kazuya Browse this author | | Hasegawa, Eisuke Browse this author →KAKEN DB |
| Keywords: | genomic DNA | | inbreeding | | Menderian inheritance | | reproductive mode |
| Issue Date: | 18-Apr-2013 |
| Publisher: | University of Arizona Library |
| Journal Title: | Journal of Insect Science |
| Volume: | 13 |
| Issue: | 30 |
| Start Page: | 1 |
| End Page: | 5 |
| Publisher DOI: | 10.1673/031.013.3001 |
| PMID: | 23902377 |
| Abstract: | Thrips tabaci Lindeman (Thysanoptera: Thripidae), a cosmopolitan pest insect, is subdivided into at least three genetic lineages that have different biological characters, such as reproductive mode and resistibility to insecticides. Since the lineages are discriminated only by mitochondrial DNA, there is a possibility of gene flow among lineages at the genomic level. Nine polymorphic microsatellite loci were newly isolated from the taxon. Moderate to high levels of polymorphism were observed, with numbers of alleles ranging from 6 to 12 in 51 individuals. The mean observed and expected heterozygosities ranged from 0.1373 to 0.3725 and 0.5381 to 0.7748, respectively. Contrary to the expectation under Hardy-Weinberg’s equilibrium, six of the nine loci exhibited a reduction to homozygosities. However, we confirmed that alleles in all the loci were inherited as Mendeilan characteristics. These new loci will be useful to explore discrimination of lineages and population genetics in this species. |
| Rights: | http://creativecommons.org/licenses/by/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/66453 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
|
Submitter: 長谷川 英祐
|
