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Hokkaido University Collection of Scholarly and Academic Papers >
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Development of a serodiagnostic multi-species ELISA against tick-borne encephalitis virus using subviral particles
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| Title: | Development of a serodiagnostic multi-species ELISA against tick-borne encephalitis virus using subviral particles |
| Authors: | Inagaki, Eri Browse this author | | Sakai, Mizuki Browse this author | | Hirano, Minato Browse this author | | Muto, Memi Browse this author | | Kobayashi, Shintaro Browse this author →KAKEN DB | | Kariwa, Hiroaki Browse this author →KAKEN DB | | Yoshii, Kentaro Browse this author →KAKEN DB |
| Keywords: | Tick-borne encephalitis virus | | Flavivirus | | Subviral particles | | ELISA |
| Issue Date: | Jul-2016 |
| Publisher: | Elsevier |
| Journal Title: | Ticks and Tick-Borne Diseases |
| Volume: | 7 |
| Issue: | 5 |
| Start Page: | 723 |
| End Page: | 729 |
| Publisher DOI: | 10.1016/j.ttbdis.2016.03.002 |
| PMID: | 26969490 |
| Abstract: | Tick-borne encephalitis virus (TBEV) is a zoonotic agent causing severe encephalitis in humans. A wide range of animal species could be infected with TBEV in endemic areas. A serological survey of wild animals is effective in identifying TBEV-endemic areas. Safe, simple, and reliable TBEV serodiagnostic tools are needed to test animals. In this study, ELISA was developed to detect anti-TBEV specific antibodies in multi-species of animals, using recombinant subviral particles (SPs) with an affinity tag and protein A/G. A Strep-tag was fused at the N terminus of the E protein of the plasmid coding TBEV prME. The E proteins with Strep-tag were secreted as SPs, of which Strep-tag was exposed on the surface. The tagged E proteins were associated with prM. The SPs with Strep-tag were applied as the antigen of ELISA, and TBEV-specific antibodies were detected by the protein A/G. Compared to neutralization test results, the ELISA showed 96.8% sensitivity and 97.7% specificity in rodents and 95.1% sensitivity and 96.0% specificity in humans, without cross-reactivity with antibodies to Japanese encephalitis virus. These results indicate that our ELISA would be useful to detect TBE-specific antibodies in a wide range of animal species. (C) 2016 Elsevier GmbH. All rights reserved. |
| Rights: | © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/ | | http://creativecommons.org/licenses/by-nc-nd/4.0/ |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/66556 |
| Appears in Collections: | 獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 好井 健太朗
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