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Development of a serodiagnostic multi-species ELISA against tick-borne encephalitis virus using subviral particles

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Ticks Tick Borne Dis_v.7 p.723–729_2016.pdf254.55 kBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/66556

Title: Development of a serodiagnostic multi-species ELISA against tick-borne encephalitis virus using subviral particles
Authors: Inagaki, Eri Browse this author
Sakai, Mizuki Browse this author
Hirano, Minato Browse this author
Muto, Memi Browse this author
Kobayashi, Shintaro Browse this author →KAKEN DB
Kariwa, Hiroaki Browse this author →KAKEN DB
Yoshii, Kentaro Browse this author →KAKEN DB
Keywords: Tick-borne encephalitis virus
Flavivirus
Subviral particles
ELISA
Issue Date: Jul-2016
Publisher: Elsevier
Journal Title: Ticks and Tick-Borne Diseases
Volume: 7
Issue: 5
Start Page: 723
End Page: 729
Publisher DOI: 10.1016/j.ttbdis.2016.03.002
PMID: 26969490
Abstract: Tick-borne encephalitis virus (TBEV) is a zoonotic agent causing severe encephalitis in humans. A wide range of animal species could be infected with TBEV in endemic areas. A serological survey of wild animals is effective in identifying TBEV-endemic areas. Safe, simple, and reliable TBEV serodiagnostic tools are needed to test animals. In this study, ELISA was developed to detect anti-TBEV specific antibodies in multi-species of animals, using recombinant subviral particles (SPs) with an affinity tag and protein A/G. A Strep-tag was fused at the N terminus of the E protein of the plasmid coding TBEV prME. The E proteins with Strep-tag were secreted as SPs, of which Strep-tag was exposed on the surface. The tagged E proteins were associated with prM. The SPs with Strep-tag were applied as the antigen of ELISA, and TBEV-specific antibodies were detected by the protein A/G. Compared to neutralization test results, the ELISA showed 96.8% sensitivity and 97.7% specificity in rodents and 95.1% sensitivity and 96.0% specificity in humans, without cross-reactivity with antibodies to Japanese encephalitis virus. These results indicate that our ELISA would be useful to detect TBE-specific antibodies in a wide range of animal species. (C) 2016 Elsevier GmbH. All rights reserved.
Rights: © 2016. This manuscript version is made available under the CC-BY-NC-ND 4.0 license http://creativecommons.org/licenses/by-nc-nd/4.0/
http://creativecommons.org/licenses/by-nc-nd/4.0/
Type: article (author version)
URI: http://hdl.handle.net/2115/66556
Appears in Collections:獣医学院・獣医学研究院 (Graduate School of Veterinary Medicine / Faculty of Veterinary Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 好井 健太朗

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