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Decreased RNA-binding motif 5 expression is associated with tumor progression in gastric cancer
This item is licensed under:Creative Commons Attribution-NonCommercial 3.0 Unported
| Title: | Decreased RNA-binding motif 5 expression is associated with tumor progression in gastric cancer |
| Authors: | Kobayashi, Takahiko Browse this author →KAKEN DB | | Ishida, Junich Browse this author | | Shimizu, Yuichi Browse this author →KAKEN DB | | Kawakami, Hiroshi Browse this author →KAKEN DB | | Suda, Goki Browse this author →KAKEN DB | | Muranaka, Tetsuhito Browse this author | | Komatsu, Yoshito Browse this author →KAKEN DB | | Asaka, Masahiro Browse this author →KAKEN DB | | Sakamoto, Naoya Browse this author →KAKEN DB |
| Keywords: | Gastric cancer | | RNA-binding motif 5 | | tumor suppressor gene | | tumor progression |
| Issue Date: | 1-Mar-2017 |
| Publisher: | SAGE Publications |
| Journal Title: | Tumor biology |
| Volume: | 39 |
| Issue: | 3 |
| Publisher DOI: | 10.1177/1010428317694547 |
| PMID: | 28347247 |
| Abstract: | RNA-binding motif 5 is a putative tumor suppressor gene that modulates cell cycle arrest and apoptosis. We recently demonstrated that RNA-binding motif 5 inhibits cell growth through the p53 pathway. This study evaluated the clinical significance of RNA-binding motif 5 expression in gastric cancer and the effects of altered RNA-binding motif 5 expression on cancer biology in gastric cancer cells. RNA-binding motif 5 protein expression was evaluated by immunohistochemistry using the surgical specimens of 106 patients with gastric cancer. We analyzed the relationships of RNA-binding motif 5 expression with clinicopathological parameters and patient prognosis. We further explored the effects of RNA-binding motif 5 downregulation with short hairpin RNA on cell growth and p53 signaling in MKN45 gastric cancer cells. Immunohistochemistry revealed that RNA-binding motif 5 expression was decreased in 29 of 106 (27.4%) gastric cancer specimens. Decreased RNA-binding motif 5 expression was correlated with histological differentiation, depth of tumor infiltration, nodal metastasis, tumor-node-metastasis stage, and prognosis. RNA-binding motif 5 silencing enhanced gastric cancer cell proliferation and decreased p53 transcriptional activity in reporter gene assays. Conversely, restoration of RNA-binding motif 5 expression suppressed cell growth and recovered p53 transactivation in RNA-binding motif 5-silenced cells. Furthermore, RNA-binding motif 5 silencing reduced the messenger RNA and protein expression of the p53 target gene p21. Our results suggest that RNA-binding motif 5 downregulation is involved in gastric cancer progression and that RNA-binding motif 5 behaves as a tumor suppressor gene in gastric cancer. |
| Rights: | http://creativecommons.org/licenses/by-nc/3.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/66569 |
| Appears in Collections: | 医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 坂本 直哉
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