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Decreased RNA-binding motif 5 expression is associated with tumor progression in gastric cancer

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Title: Decreased RNA-binding motif 5 expression is associated with tumor progression in gastric cancer
Authors: Kobayashi, Takahiko Browse this author →KAKEN DB
Ishida, Junich Browse this author
Shimizu, Yuichi Browse this author →KAKEN DB
Kawakami, Hiroshi Browse this author →KAKEN DB
Suda, Goki Browse this author →KAKEN DB
Muranaka, Tetsuhito Browse this author
Komatsu, Yoshito Browse this author →KAKEN DB
Asaka, Masahiro Browse this author →KAKEN DB
Sakamoto, Naoya Browse this author →KAKEN DB
Keywords: Gastric cancer
RNA-binding motif 5
tumor suppressor gene
tumor progression
Issue Date: Mar-2017
Publisher: SAGE Publications
Journal Title: Tumor biology
Volume: 39
Issue: 3
Publisher DOI: 10.1177/1010428317694547
PMID: 28347247
Abstract: RNA-binding motif 5 is a putative tumor suppressor gene that modulates cell cycle arrest and apoptosis. We recently demonstrated that RNA-binding motif 5 inhibits cell growth through the p53 pathway. This study evaluated the clinical significance of RNA-binding motif 5 expression in gastric cancer and the effects of altered RNA-binding motif 5 expression on cancer biology in gastric cancer cells. RNA-binding motif 5 protein expression was evaluated by immunohistochemistry using the surgical specimens of 106 patients with gastric cancer. We analyzed the relationships of RNA-binding motif 5 expression with clinicopathological parameters and patient prognosis. We further explored the effects of RNA-binding motif 5 downregulation with short hairpin RNA on cell growth and p53 signaling in MKN45 gastric cancer cells. Immunohistochemistry revealed that RNA-binding motif 5 expression was decreased in 29 of 106 (27.4%) gastric cancer specimens. Decreased RNA-binding motif 5 expression was correlated with histological differentiation, depth of tumor infiltration, nodal metastasis, tumor-node-metastasis stage, and prognosis. RNA-binding motif 5 silencing enhanced gastric cancer cell proliferation and decreased p53 transcriptional activity in reporter gene assays. Conversely, restoration of RNA-binding motif 5 expression suppressed cell growth and recovered p53 transactivation in RNA-binding motif 5-silenced cells. Furthermore, RNA-binding motif 5 silencing reduced the messenger RNA and protein expression of the p53 target gene p21. Our results suggest that RNA-binding motif 5 downregulation is involved in gastric cancer progression and that RNA-binding motif 5 behaves as a tumor suppressor gene in gastric cancer.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 坂本 直哉

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