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The amino acid at position 624 in the glycoprotein of SFTSV (severe fever with thrombocytopenia virus) plays a critical role in low-pH-dependent cell fusion activity

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Title: The amino acid at position 624 in the glycoprotein of SFTSV (severe fever with thrombocytopenia virus) plays a critical role in low-pH-dependent cell fusion activity
Authors: Tsuda, Yoshimi Browse this author
Igarashi, Manabu Browse this author →KAKEN DB
Ito, Ryo Browse this author
Nishio, Sanae Browse this author
Shimizu, Kenta Browse this author →KAKEN DB
Yoshimatsu, Kumiko Browse this author
Arikawa, Jiro Browse this author →KAKEN DB
Issue Date: 2017
Publisher: Biomedical Research Press
Journal Title: Biomedical Research
Volume: 38
Issue: 2
Start Page: 89
End Page: 97
Publisher DOI: 10.2220/biomedres.38.89
PMID: 28442665
Abstract: Severe fever with thrombocytopenia syndrome virus (SFTSV) is a novel phlebovirus responsible for causing an emerging zoonotic disease. We previously established subclones from SFTSV strain YG1 based on differences in low-pH-dependent cell fusion activities and found two amino acid substitutions, Y328H and R624W, in the envelope glycoprotein (GP) of high fusion subclones. In this study, we show that transiently expressed GP with the R624W mutation, but not the Y328H mutation, induced cell fusion under acidic conditions. GP possessing either tryptophan, serine, glycine or aspartic acid at position 624 induced cell fusion, whereas GP possessing basic amino acids such as arginine or lysine did not induce cell fusion. These results indicated that the amino acid at position 624 has an important role for inducing low-pH-dependent cell fusion.
Type: article
URI: http://hdl.handle.net/2115/66590
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 津田 祥美

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