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Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress

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Title: Protective effects and functional mechanisms of Lactobacillus gasseri SBT2055 against oxidative stress
Authors: Kobatake, Eiji Browse this author
Nakagawa, Hisako Browse this author
Seki, Takahiro Browse this author
Miyazaki, Tadaaki Browse this author →KAKEN DB
Issue Date: 11-May-2017
Publisher: Public Library of Science
Journal Title: PLoS ONE
Volume: 12
Issue: 5
Start Page: e0177106
Publisher DOI: 10.1371/journal.pone.0177106
Abstract: Lactobacillus gasseri SBT2055 (LG2055) is one of the probiotic lactic acid bacteria. Recently, we demonstrated that feeding with LG2055 extended the lifespan of Caenorhabditis elegans and that the prolongevity effect was dependent upon the regulation of oxidative stress response. In this study, we assessed whether LG2055 regulated the oxidative stress response of mammalian cells. In NIH-3T3 cells and primary mouse embryonic fibroblast cells, low cell proliferation rates and high reactive oxygen species levels were observed following paraquat treatment. LG2055 treatment suppressed these responses in paraquat-treated cells, indicating that LG2055 protected against oxidative stress in mammalian cells. The mRNA expression of oxidative stress-related genes, total nuclear factor-erythroid-2-related factor 2 (Nrf2) protein levels, and the nuclear translocation of Nrf2 were increased by LG2055 treatment. These results suggested that the Nrf2-antioxidant response element (ARE) signaling pathway was activated by LG2055. Furthermore, c-Jun NH2-terminal kinase (JNK) was activated by LG2055 treatment and the inhibition of JNK suppressed the activation of the Nrf2-ARE signaling pathway in LG2055-treated cells. Together, these findings suggest that LG2055 activated the Nrf2-ARE signaling pathway by JNK activation, thus strengthening the defense system against oxidative stress in mammalian cells.
Type: article
Appears in Collections:遺伝子病制御研究所 (Institute for Genetic Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宮崎 忠昭

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