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Circulating osteocyte-derived exosomes contain miRNAs which are enriched in exosomes from MLO-Y4 cells

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Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/66897

Title: Circulating osteocyte-derived exosomes contain miRNAs which are enriched in exosomes from MLO-Y4 cells
Authors: Sato, Mari Browse this author →KAKEN DB
Suzuki, Tomohide Browse this author
Kawano, Mitsuoki Browse this author →KAKEN DB
Tamura, Masato Browse this author →KAKEN DB
Keywords: osteocyte
exosome
microRNA
circulating exosome
Issue Date: Feb-2017
Publisher: Spandidos Publications
Journal Title: Biomedical reports
Volume: 6
Issue: 2
Start Page: 223
End Page: 231
Publisher DOI: 10.3892/br.2016.824
PMID: 28357077
Abstract: Signaling molecules produced by osteocytes have been proposed to serve as soluble factors that contribute to bone remodeling, as well as to homeostasis of other organs. However, to the best of our knowledge, there are currently no studies investigating the role of osteocyte-secreted exosomes. In the present study, ablation of osteocytes in mice [osteocyte-less (OL)] was used to examine the microRNA (miRNA) levels of plasma-circulating exosomes. In order to investigate the function of osteocyte-secreted exosomes, exosomes derived from MLO-Y4 cells were extracted and their miRNA expression levels were examined using miRNA array analysis and deep sequencing. Comparison of miRNA expression levels between plasma exosomes from OL mouse plasma and MLO-Y4-derived exosomes revealed that decreases in the number of miRNAs from exosomes circulating in the OL mouse plasma may be caused by a decrease in secretion of exosomes from osteocytes. These results suggest that osteocytes secrete exosomes containing characterized miRNAs and then circulate in the blood, and may thus transfer their components, including miRNAs, to recipient cells where they function as signaling molecules in other organs and/ or tissues to regulate biological responses.
Type: article
URI: http://hdl.handle.net/2115/66897
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 田村 正人

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