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Establishment of a rat model of thrombosis induced by intravenous injection of anti-phosphatidylserine–prothrombin complex antibody

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Title: Establishment of a rat model of thrombosis induced by intravenous injection of anti-phosphatidylserine–prothrombin complex antibody
Authors: Yamada, Mai Browse this author
Kawakami, Tamihiro Browse this author
Takashima, Kohei Browse this author
Nishioka, Yusuke Browse this author
Nishibata, Yuka Browse this author
Masuda, Sakiko Browse this author
Yoshida, Shigeru Browse this author →KAKEN DB
Tomaru, Utano Browse this author →KAKEN DB
Ishizu, Akihiro Browse this author →KAKEN DB
Keywords: anti-phosphatidylserine–prothrombin complex antibody
anti-phospholipid syndrome
thrombosis
animal model
Issue Date: Jun-2017
Publisher: Oxford University Press
Journal Title: Rheumatology
Volume: 56
Issue: 6
Start Page: 1013
End Page: 1018
Publisher DOI: 10.1093/rheumatology/kew477
PMID: 28073955
Abstract: Objective. Recent studies have suggested that aPS-PT antibody is one of the most relevant autoantibodies to APS. This study aimed to demonstrate the pathogenicity of aPS-PT antibody in vivo. Methods. At first, cultured rat vascular endothelial cells (RECs) were exposed to calf thymus-derived histones. Two hours later, lactate dehydrogenase release from the RECs and expression of PS on the cell surface were assessed. Next, we administered an i.v. injection of calf thymus-derived histones into Wistar rats (12.5 µg/g weight of 8-week-old female rats), and 2 h later they were given an i.v. injection of aPS-PT mAb (1.25 mg/g weight, n = 6) or an equal dose of rat IgM as controls (n = 5). Three days later, histological examination was conducted. Results. Calf thymus-derived histones (>12.5 µg/ml) could injure RECs in vitro. Simultaneously, annexin V could bind to the RECs; thereby, this result indicated that cell-free histone exposure of vascular endothelial cells induced cell surface expression of PS, which is naturally present inside the plasma membrane. Thrombosis developed with higher frequency in the rats given an i.v. injection of aPS-PT mAb than in controls. Conclusion. We established a rat model of thrombosis induced by i.v. injection of aPS-PT mAb.
Rights: http://creativecommons.org/licenses/by-nc/4.0/
Type: article
URI: http://hdl.handle.net/2115/66949
Appears in Collections:保健科学院・保健科学研究院 (Graduate School of Health Sciences / Faculty of Health Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 石津 明洋

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