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Establishment of a rat model of thrombosis induced by intravenous injection of anti-phosphatidylserine–prothrombin complex antibody

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タイトル: Establishment of a rat model of thrombosis induced by intravenous injection of anti-phosphatidylserine–prothrombin complex antibody
著者: Yamada, Mai 著作を一覧する
Kawakami, Tamihiro 著作を一覧する
Takashima, Kohei 著作を一覧する
Nishioka, Yusuke 著作を一覧する
Nishibata, Yuka 著作を一覧する
Masuda, Sakiko 著作を一覧する
Yoshida, Shigeru 著作を一覧する
Tomaru, Utano 著作を一覧する
Ishizu, Akihiro 著作を一覧する
キーワード: anti-phosphatidylserine–prothrombin complex antibody
anti-phospholipid syndrome
thrombosis
animal model
発行日: 2017年 6月
出版者: Oxford University Press
誌名: Rheumatology
巻: 56
号: 6
開始ページ: 1013
終了ページ: 1018
出版社 DOI: 10.1093/rheumatology/kew477
抄録: Objective. Recent studies have suggested that aPS-PT antibody is one of the most relevant autoantibodies to APS. This study aimed to demonstrate the pathogenicity of aPS-PT antibody in vivo. Methods. At first, cultured rat vascular endothelial cells (RECs) were exposed to calf thymus-derived histones. Two hours later, lactate dehydrogenase release from the RECs and expression of PS on the cell surface were assessed. Next, we administered an i.v. injection of calf thymus-derived histones into Wistar rats (12.5 µg/g weight of 8-week-old female rats), and 2 h later they were given an i.v. injection of aPS-PT mAb (1.25 mg/g weight, n = 6) or an equal dose of rat IgM as controls (n = 5). Three days later, histological examination was conducted. Results. Calf thymus-derived histones (>12.5 µg/ml) could injure RECs in vitro. Simultaneously, annexin V could bind to the RECs; thereby, this result indicated that cell-free histone exposure of vascular endothelial cells induced cell surface expression of PS, which is naturally present inside the plasma membrane. Thrombosis developed with higher frequency in the rats given an i.v. injection of aPS-PT mAb than in controls. Conclusion. We established a rat model of thrombosis induced by i.v. injection of aPS-PT mAb.
Relation (URI): https://academic.oup.com/rheumatology/article-lookup/doi/10.1093/rheumatology/kew477
資料タイプ: article
URI: http://hdl.handle.net/2115/66949
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 石津 明洋

 

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