HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Agriculture / Faculty of Agriculture >
Peer-reviewed Journal Articles, etc >

Two Novel Glycoside Hydrolases Responsible for the Catabolism of Cyclobis-(1→6)-α-nigerosyl

Files in This Item:
J. Biol. Chem291_16438-16447.pdf1.44 MBPDFView/Open
Please use this identifier to cite or link to this item:http://hdl.handle.net/2115/66973

Title: Two Novel Glycoside Hydrolases Responsible for the Catabolism of Cyclobis-(1→6)-α-nigerosyl
Authors: Tagami, Takayoshi Browse this author
Miyano, Eri Browse this author
Sadahiro, Juri Browse this author
Okuyama, Masayuki Browse this author →KAKEN DB
Iwasaki, Tomohito Browse this author
Kimura, Atsuo Browse this author →KAKEN DB
Keywords: actinobacteria
bacterial metabolism
carbohydrate metabolism
enzyme catalysis
enzyme kinetics
gene expression
glycoside hydrolase
glycosyltransferase
cyclic oligosaccharide
starch
Issue Date: 5-Aug-2016
Publisher: American Society for Biochemistry and Molecular Biology (ASBMB)
Journal Title: Journal of Biological Chemistry (JBC)
Volume: 291
Issue: 32
Start Page: 16438
End Page: 16447
Publisher DOI: 10.1074/jbc.M116.727305
PMID: 27302067
Abstract: The actinobacterium Kribbella flavida NBRC 14399(T) produces cyclobis-(1 -> 6)-alpha-nigerosyl (CNN), a cyclic glucotetraose with alternate alpha-(1 -> 6)- and alpha-(1 -> 3)-glucosidic linkages, from starch in the culture medium. We identified gene clusters associated with the production and intracellular catabolism of CNN in the K. flavida genome. One cluster encodes 6-alpha-glucosyl-transferase and 3-alpha-isomaltosyltransferase, which are known to coproduce CNN from starch. The other cluster contains four genes annotated as a transcriptional regulator, sugar transporter, glycoside hydrolase family (GH) 31 protein (Kfla1895), and GH15 protein (Kfla1896). Kfla1895 hydrolyzed the alpha-(1 -> 3)-glucosidic linkages of CNN and produced isomaltose via a possible linear tetrasaccharide. The initial rate of hydrolysis of CNN (11.6 s(-1)) was much higher than that of panose (0.242 s(-1)), and hydrolysis of isomaltotriose and nigerose was extremely low. Because Kfla1895 has a strong preference for the alpha-(1 -> 3)-isomaltosyl moiety and effectively hydrolyzes the alpha-(1 -> 3)-glucosidic linkage, it should be termed 1,3-alpha-isomaltosidase. Kfla1896 effectively hydrolyzed isomaltose with liberation of beta-glucose, but displayed low or no activity toward CNN and the general GH15 enzyme substrates such as maltose, soluble starch, or dextran. The k(cat)/K-m for isomaltose (4.81 +/- 0.18 s(-1) mM(-1)) was 6.9- and 19-fold higher than those for panose and isomaltotriose, respectively. These results indicate that Kfla1896 is a new GH15 enzyme with high substrate specificity for isomaltose, suggesting the enzyme should be designated an isomaltose glucohydrolase. This is the first report to identify a starch-utilization pathway that proceeds via CNN.
Rights: This research was originally published in Journal of Biological Chemistry (JBC). Tagami, Takayoshi; Miyano, Eri; Sadahiro, Juri; Okuyama, Masayuki; Iwasaki, Tomohito; Kimura, Atsuo. Two Novel Glycoside Hydrolases Responsible for the Catabolism of Cyclobis-(1→6)-α-nigerosyl. Journal of Biological Chemistry (JBC). 2016; Vol:pp.16438-16447.©the American Society for Biochemistry and Molecular Biology.
Type: article
URI: http://hdl.handle.net/2115/66973
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 木村 淳夫

Export metadata:

OAI-PMH ( junii2 , jpcoar )


 

Feedback - Hokkaido University