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Elucidation of the biosynthetic pathway of cis-jasmone in Lasiodiplodia theobromae


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タイトル: Elucidation of the biosynthetic pathway of cis-jasmone in Lasiodiplodia theobromae
著者: Matsui, Ryo 著作を一覧する
Amano, Naruki 著作を一覧する
Takahashi, Kosaku 著作を一覧する
Taguchi, Yodai 著作を一覧する
Saburi, Wataru 著作を一覧する
Mori, Hideharu 著作を一覧する
Kondo, Norio 著作を一覧する
Matsuda, Kazuhiko 著作を一覧する
Matsuura, Hideyuki 著作を一覧する
キーワード: Bioinorganic chemistry
Secondary metabolism
発行日: 2017年 7月27日
出版者: Nature Publishing Group
誌名: Scientific reports
巻: 7
号: 1
開始ページ: 6688
出版社 DOI: 10.1038/s41598-017-05851-7
抄録: In plants, cis-jasmone (CJ) is synthesized from a-linolenic acid (LA) via two biosynthetic pathways using jasmonic acid (JA) and iso-12-oxo-phytodienoic acid (iso-OPDA) as key intermediates. However, there have been no reports documenting CJ production by microorganisms. In the present study, the production of fungal-derived CJ by Lasiodiplodia theobromae was observed for the first time, although this production was not observed for Botrytis cinerea, Verticillium longisporum, Fusarium oxysporum, Gibberella fujikuroi, and Cochliobolus heterostrophus. To investigate the biosynthetic pathway of CJ in L. theobromae, administration experiments using [18,18,18-²H₃, 17,17-²H₂] LA (LA-d5), [18,18,18-²H₃, 17,17-²H₂]12-oxo-phytodienoic acid (cis-OPDA-d5), [5', 5', 5'-²H₃, 4', 4'-²H₂, 3'-²H₁] OPC 8:0 (OPC8-d6), [5', 5', 5'-²H₃, 4', 4'-²H₂, 3'-²H₁] OPC 6:0 (OPC6-d6), [5', 5', 5'-²H₃, 4', 4'-²H₂, 3'-²H₁] OPC 4:0 (OPC4-d6), and [11,11-²H₂, 10,10-²H₂, 8,8-²H₂, 2,2-²H₂] methyl iso-12-oxo-phytodienoate (iso-MeOPDA-d8) were carried out, revealing that the fungus produced CJ through a single biosynthetic pathway via iso-OPDA. Interestingly, it was suggested that the previously predicted decarboxylation step of 3,7-didehydroJA to afford CJ might not be involved in CJ biosynthesis in L. theobromae.
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 松浦 英幸


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