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Aquaporin-mediated long-distance polyphosphate translocation directed towards the host in arbuscular mycorrhizal symbiosis: application of virus-induced gene silencing

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タイトル: Aquaporin-mediated long-distance polyphosphate translocation directed towards the host in arbuscular mycorrhizal symbiosis: application of virus-induced gene silencing
著者: Kikuchi, Yusuke 著作を一覧する
Hijikata, Nowaki 著作を一覧する
Ohtomo, Ryo 著作を一覧する
Handa, Yoshihiro 著作を一覧する
Kawaguchi, Masayoshi 著作を一覧する
Saito, Katsuharu 著作を一覧する
Masuta, Chikara 著作を一覧する
Ezawa, Tatsuhiro 著作を一覧する
キーワード: arbuscular mycorrhiza
aquaglyceroporin
aquaporin
cucumber mosaic virus
polyphosphate translocation
Rhizophagus clarus, transpiration
virus-induced gene silencing
発行日: 2016年 9月
出版者: Wiley-Blackwell
誌名: New Phytologist
巻: 211
号: 4
開始ページ: 1202
終了ページ: 1208
出版社 DOI: 10.1111/nph.14016
抄録: Arbuscular mycorrhizal fungi translocate polyphosphate through hyphae over a long distance to deliver to the host. More than three decades ago, suppression of host transpiration was found to decelerate phosphate delivery of the fungal symbiont, leading us to hypothesize that transpiration provides a primary driving force for polyphosphate translocation, probably via creating hyphal water flow in which fungal aquaporin(s) may be involved. The impact of transpiration suppression on polyphosphate translocation through hyphae of Rhizophagus clarus was evaluated. An aquaporin gene expressed in intraradical mycelia was characterized and knocked down by virus-induced gene silencing to investigate the involvement of the gene in polyphosphate translocation. Rhizophagus clarus aquaporin 3 (RcAQP3) that was most highly expressed in intraradical mycelia encodes an aquaglyceroporin responsible for water transport across the plasma membrane. Knockdown of RcAQP3 as well as the suppression of host transpiration decelerated polyphosphate translocation in proportion to the levels of knockdown and suppression, respectively. These results provide the first insight into the mechanism underlying long-distance polyphosphate translocation in mycorrhizal associations at the molecular level, in which host transpiration and the fungal aquaporin play key roles. A hypothetical model of the translocation is proposed for further elucidation of the mechanism.
Rights: This is the peer reviewed version of the following article: [Aquaporin-mediated long-distance polyphosphate translocation directed towards the host in arbuscular mycorrhizal symbiosis : application of virus-induced gene silencing], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/67113
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 江澤 辰広

 

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