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Aquaporin-mediated long-distance polyphosphate translocation directed towards the host in arbuscular mycorrhizal symbiosis: application of virus-induced gene silencing
| Title: | Aquaporin-mediated long-distance polyphosphate translocation directed towards the host in arbuscular mycorrhizal symbiosis: application of virus-induced gene silencing |
| Authors: | Kikuchi, Yusuke Browse this author | | Hijikata, Nowaki Browse this author | | Ohtomo, Ryo Browse this author | | Handa, Yoshihiro Browse this author | | Kawaguchi, Masayoshi Browse this author | | Saito, Katsuharu Browse this author | | Masuta, Chikara Browse this author | | Ezawa, Tatsuhiro Browse this author →KAKEN DB |
| Keywords: | arbuscular mycorrhiza | | aquaglyceroporin | | aquaporin | | cucumber mosaic virus | | polyphosphate translocation | | Rhizophagus clarus, transpiration | | virus-induced gene silencing |
| Issue Date: | Sep-2016 |
| Publisher: | Wiley-Blackwell |
| Journal Title: | New Phytologist |
| Volume: | 211 |
| Issue: | 4 |
| Start Page: | 1202 |
| End Page: | 1208 |
| Publisher DOI: | 10.1111/nph.14016 |
| PMID: | 27136716 |
| Abstract: | Arbuscular mycorrhizal fungi translocate polyphosphate through hyphae over a long distance to deliver to the host. More than three decades ago, suppression of host transpiration was found to decelerate phosphate delivery of the fungal symbiont, leading us to hypothesize that transpiration provides a primary driving force for polyphosphate translocation, probably via creating hyphal water flow in which fungal aquaporin(s) may be involved. The impact of transpiration suppression on polyphosphate translocation through hyphae of Rhizophagus clarus was evaluated. An aquaporin gene expressed in intraradical mycelia was characterized and knocked down by virus-induced gene silencing to investigate the involvement of the gene in polyphosphate translocation. Rhizophagus clarus aquaporin 3 (RcAQP3) that was most highly expressed in intraradical mycelia encodes an aquaglyceroporin responsible for water transport across the plasma membrane. Knockdown of RcAQP3 as well as the suppression of host transpiration decelerated polyphosphate translocation in proportion to the levels of knockdown and suppression, respectively. These results provide the first insight into the mechanism underlying long-distance polyphosphate translocation in mycorrhizal associations at the molecular level, in which host transpiration and the fungal aquaporin play key roles. A hypothetical model of the translocation is proposed for further elucidation of the mechanism. |
| Rights: | This is the peer reviewed version of the following article: [Aquaporin-mediated long-distance polyphosphate translocation directed towards the host in arbuscular mycorrhizal symbiosis : application of virus-induced gene silencing], which has been published in final form at [Link to final article using the DOI]. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/67113 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 江澤 辰広
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