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Volume 65 Number 3 >

Effects of semen preservation procedure in egg yolk-tris based extender on bull spermatozoa characteristics

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Please use this identifier to cite or link to this item:http://doi.org/10.14943/jjvr.65.3.151

Title: Effects of semen preservation procedure in egg yolk-tris based extender on bull spermatozoa characteristics
Authors: Kanno, Chihiro Browse this author
Yanagawa, Yojiro Browse this author
Takahashi, Yoshiyuki Browse this author
Katagiri, Seiji Browse this author
Nagano, Masashi Browse this author
Keywords: acrosome
mitochondrial membrane potential
semen preservation
Issue Date: Aug-2017
Publisher: Faculty of Veterinary Medicine, Hokkaido University
Journal Title: Japanese Journal of Veterinary Research
Volume: 65
Issue: 3
Start Page: 151
End Page: 157
Abstract: To verify the dynamics of damages to spermatozoa during semen freezing, characteristics of spermatozoa collected from 3 Japanese black bulls were evaluated by using fluorescent staining. Pre-diluted sample showed the highest proportion of spermatozoa with intact plasma membrane, intact acrosome and high mitochondrial potential. The proportion of spermatozoa with intact plasma membrane, intact acrosome, and low mitochondrial membrane potential were higher after cooling to 4°C than the other processes. During cooling preservation examined in this study, the proportion of spermatozoa with damaged acrosome increased. These results lead us to speculate that, during cooling process, spermatozoa may be firstly injured to mitochondrial membrane, and low mitochondrial function may cause the impairment of plasma membrane and subsequent cell death with acrosomal damage.
Type: bulletin (article)
URI: http://hdl.handle.net/2115/67143
Appears in Collections:Japanese Journal of Veterinary Research > Volume 65 Number 3

Submitter: 獣医学部図書室

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