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Synchronous circadian voltage rhythms with asynchronous calcium rhythms in the suprachiasmatic nucleus

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Title: Synchronous circadian voltage rhythms with asynchronous calcium rhythms in the suprachiasmatic nucleus
Authors: Enoki, Ryosuke Browse this author →KAKEN DB
Oda, Yoshiaki Browse this author
Mieda, Michihiro Browse this author
Ono, Daisuke Browse this author →KAKEN DB
Honma, Sato Browse this author →KAKEN DB
Honma, Ken-ichi Browse this author →KAKEN DB
Keywords: circadian rhythm
membrane potential
intracellular calcium
time-lapse imaging
neuronal network
Issue Date: 21-Mar-2017
Publisher: National Academy of Sciences
Journal Title: Proceedings of the National Academy of Sciences of the United States of America
Volume: 114
Issue: 12
Start Page: E2476
End Page: E2485
Publisher DOI: 10.1073/pnas.1616815114
Abstract: The suprachiasmatic nucleus (SCN), the master circadian clock, contains a network composed of multiple types of neurons which are thought to form a hierarchical and multioscillator system. The molecular clock machinery in SCN neurons drives membrane excitability and sends time cue signals to various brain regions and peripheral organs. However, how and at what time of the day these neurons transmit output signals remain largely unknown. Here, we successfully visualized circadian voltage rhythms optically for many days using a genetically encoded voltage sensor, ArcLightD. Unexpectedly, the voltage rhythms are synchronized across the entire SCN network of cultured slices, whereas simultaneously recorded Ca2+ rhythms are topologically specific to the dorsal and ventral regions. We further found that the temporal order of these two rhythms is cell-type specific: The Ca2+ rhythms phase-lead the voltage rhythms in AVP neurons but Ca2+ and voltage rhythms are nearly in phase in VIP neurons. We confirmed that circadian firing rhythms are also synchronous and are coupled with the voltage rhythms. These results indicate that SCN networks with asynchronous Ca2+ rhythms produce coherent voltage rhythms.
Type: article (author version)
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 榎木 亮介

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