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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences >
Peer-reviewed Journal Articles, etc >
Isolation and characterization of a new DUR3-like gene, PyDUR3.3, from the marine macroalga Pyropia yezoensis (Rhodophyta)
| Title: | Isolation and characterization of a new DUR3-like gene, PyDUR3.3, from the marine macroalga Pyropia yezoensis (Rhodophyta) |
| Authors: | Kakinuma, Makoto Browse this author | | Suzuki, Kohei Browse this author | | Iwata, Shintaro Browse this author | | Coury, Daniel A. Browse this author | | Iwade, Shouei Browse this author | | Mikami, Koji Browse this author →KAKEN DB |
| Keywords: | cDNA cloning | | DUR3 | | Gene expression | | Pyropia yezoensis | | Rhodophyta | | Sodium solute symporter | | Urea transporter |
| Issue Date: | Jan-2016 |
| Publisher: | 日本水産学会 |
| Journal Title: | Fisheries Science |
| Volume: | 82 |
| Issue: | 1 |
| Start Page: | 171 |
| End Page: | 184 |
| Publisher DOI: | 10.1007/s12562-015-0947-7 |
| Abstract: | DUR3 proteins mediate high-affinity transport of exogenous and endogenous urea. Although two DUR3-like genes (PyDUR3.1/3.2) have been identified in Pyropia yezoensis, a BLAST search using these sequences against the P. yezoensis EST database suggested the existence of another DUR3-like gene (PyDUR3.3). In this study, the PyDUR3.3 gene was isolated and characterized and compared to PyDUR3.1/3.2 genes. The predicted length of PyDUR3.3 was 679 amino acids, which included 15 transmembrane domains. An amino acid sequence alignment of algal, plant, and yeast DUR3 proteins showed that PyDUR3.3 was more similar to PyDUR3.2 than to other DUR3 proteins, including PyDUR3.1. Exon–intron structures of PyDUR3.3 and PyDUR3.2 were also closely related to each other, which clearly differed from that of PyDUR3.1. Expression analysis showed that PyDUR3.3 mRNA levels were extremely high in sporophytes, regardless of the nutrient condition, compared to gametophytes. On the other hand, expression of PyDUR3.2 and PyDUR3.1 was high in the gametophytes and sporophytes, respectively, and was induced by nutrient starvation. These results suggest that expression of PyDUR3.3/3.2/3.1 depends on the life history phase as well as the nutrient conditions, and that PyDUR3.3 and PyDUR3.2 are paralogues specifically differentiated in function and life history phase. |
| Rights: | The final publication is available at www.springerlink.com via http://dx.doi.org/10.1007/s12562-015-0947-7 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/67198 |
| Appears in Collections: | 水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 三上 浩司
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