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Isolation and characterization of a new DUR3-like gene, PyDUR3.3, from the marine macroalga Pyropia yezoensis (Rhodophyta)

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Title: Isolation and characterization of a new DUR3-like gene, PyDUR3.3, from the marine macroalga Pyropia yezoensis (Rhodophyta)
Authors: Kakinuma, Makoto Browse this author
Suzuki, Kohei Browse this author
Iwata, Shintaro Browse this author
Coury, Daniel A. Browse this author
Iwade, Shouei Browse this author
Mikami, Koji Browse this author →KAKEN DB
Keywords: cDNA cloning
DUR3
Gene expression
Pyropia yezoensis
Rhodophyta
Sodium solute symporter
Urea transporter
Issue Date: Jan-2016
Publisher: 日本水産学会
Journal Title: Fisheries Science
Volume: 82
Issue: 1
Start Page: 171
End Page: 184
Publisher DOI: 10.1007/s12562-015-0947-7
Abstract: DUR3 proteins mediate high-affinity transport of exogenous and endogenous urea. Although two DUR3-like genes (PyDUR3.1/3.2) have been identified in Pyropia yezoensis, a BLAST search using these sequences against the P. yezoensis EST database suggested the existence of another DUR3-like gene (PyDUR3.3). In this study, the PyDUR3.3 gene was isolated and characterized and compared to PyDUR3.1/3.2 genes. The predicted length of PyDUR3.3 was 679 amino acids, which included 15 transmembrane domains. An amino acid sequence alignment of algal, plant, and yeast DUR3 proteins showed that PyDUR3.3 was more similar to PyDUR3.2 than to other DUR3 proteins, including PyDUR3.1. Exon–intron structures of PyDUR3.3 and PyDUR3.2 were also closely related to each other, which clearly differed from that of PyDUR3.1. Expression analysis showed that PyDUR3.3 mRNA levels were extremely high in sporophytes, regardless of the nutrient condition, compared to gametophytes. On the other hand, expression of PyDUR3.2 and PyDUR3.1 was high in the gametophytes and sporophytes, respectively, and was induced by nutrient starvation. These results suggest that expression of PyDUR3.3/3.2/3.1 depends on the life history phase as well as the nutrient conditions, and that PyDUR3.3 and PyDUR3.2 are paralogues specifically differentiated in function and life history phase.
Rights: The final publication is available at www.springerlink.com via http://dx.doi.org/10.1007/s12562-015-0947-7
Type: article (author version)
URI: http://hdl.handle.net/2115/67198
Appears in Collections:水産科学院・水産科学研究院 (Graduate School of Fisheries Sciences / Faculty of Fisheries Sciences) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 三上 浩司

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