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Increased fructose 2,6-bisphosphate in peripheral blood mononuclear cells of patients with diabetes

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Title: Increased fructose 2,6-bisphosphate in peripheral blood mononuclear cells of patients with diabetes
Authors: Atsumi, Toshiya Browse this author →KAKEN DB
Chiba, Hitoshi Browse this author →KAKEN DB
Yoshioka, Narihito Browse this author →KAKEN DB
Bucala, Richard Browse this author
Koike, Takao Browse this author →KAKEN DB
Keywords: Glycolysis
Fructose 2,6-bisphosphate
Issue Date: Sep-2007
Publisher: Japan Endocrine Society
Journal Title: Endocrine journal
Volume: 54
Issue: 4
Start Page: 517
End Page: 520
Publisher DOI: 10.1507/endocrj.K06-205
Abstract: Fructose 2,6-bisphosphate (F2,6BP) is a powerful allosteric activator of 6-phosphofructo-1-kinase, which is the rate-limiting enzyme for glycolysis. Mitogenic stimulation of lymphocytes is related to an enhanced rate of glucose utilization and F2,6BP mediated activation of glycolysis. To determine the effect of hyperglycemia on intracellular glycolysis of lymphocytes, we measured intracellular F2,6BP content in peripheral blood mononuclear cells obtained from patients with diabetes and normal subjects. A total of 62 subjects participated in the present study. Venous blood samples were collected and peripheral blood mononuclear cells were separated by Ficoll gradients. Intracellular F2,6BP levels in peripheral blood mononuclear cells from normal control subjects were significantly lower than age-matched diabetic subjects. We observed a significant positive correlation between intracellular F2,6BP levels and long term glycemic control, as assessed by HbA1c. These data suggest that hyperglycemia increases intracellular F2,6BP in immune cells. These findings may help to clarify the impaired function in immune cells in patients with diabetes.
Type: article
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小池 隆夫

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