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The Influence of Polyploidy and Genome Composition on Genomic Imprinting in Mice

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Title: The Influence of Polyploidy and Genome Composition on Genomic Imprinting in Mice
Authors: Yamazaki, Wataru Browse this author
Amano, Tomoko Browse this author
Bai, Hanako Browse this author
Takahashi, Masashi Browse this author →KAKEN DB
Kawahara, Manabu Browse this author →KAKEN DB
Keywords: DNA methylation
gene expression
Issue Date: 30-Sep-2016
Publisher: American Society for Biochemistry and Molecular Biology (ASBMB)
Journal Title: Journal of Biological Chemistry (JBC)
Volume: 291
Issue: 40
Start Page: 20924
End Page: 20931
Publisher DOI: 10.1074/jbc.M116.744144
PMID: 27531747
Abstract: Genomic imprinting is an epigenetic mechanism that switches the expression of imprinted genes involved in normal embryonic growth and development in a parent-of-origin-specific manner. Changes inDNAmethylation statuses from polyploidization are a well characterized epigenetic modification in plants. However, how changes in ploidy affect both imprinted gene expression and methylation status in mammals remains unclear. To address this, we used quantitative real time PCR to analyze expression levels of imprinted genes in mouse tetraploid fetuses. We used bisulfite sequencing to assess the methylation statuses of differentially methylated regions (DMRs) that regulate imprinted gene expression in triploid and tetraploid fetuses. The nine imprinted genes H19, Gtl2, Dlk1, Igf2r, Grb10, Zim1, Peg3, Ndn, and Ipw were all unregulated; in particular, the expression of Zim1 was more than 10-fold higher, and the expression of Ipw was repressed in tetraploid fetuses. The methylation statuses of four DMRs H19, intergenic (IG), Igf2r, and Snrpn in tetraploid and triploid fetuses were similar to those in diploid fetuses. We also performed allele-specific RT-PCR sequencing to determine the alleles expressing the three imprinted genes Igf2, Gtl2, and Dlk1 in tetraploid fetuses. These three imprinted genes showed monoallelic expression in a parent-of-origin-specific manner. Expression of non-imprinted genes regulating neural cell development significantly decreased in tetraploid fetuses, which might have been associated with unregulated imprinted gene expression. This study provides the first detailed analysis of genomic imprinting in tetraploid fetuses, suggesting that imprinted gene expression is disrupted, but DNA methylation statuses of DMRs are stable following changes in ploidy in mammals.
Rights: This research was originally published in Journal of Biological Chemistry. Wataru Yamazaki, Tomoko Amano, Hanako Bai, Masashi Takahashi and Manabu Kawahara.The Influence of Polyploidy and Genome Composition on Genomic Imprinting in Mice. The Journal of Biological Chemistry. 2016; 291(40): 20924-20931. © the American Society for Biochemistry and Molecular Biology.
Type: article
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 川原 学

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