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The phenotype of infiltrating macrophages influences arteriosclerotic plaque vulnerability in the carotid artery

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タイトル: The phenotype of infiltrating macrophages influences arteriosclerotic plaque vulnerability in the carotid artery
著者: Cho, Kyu Yong 著作を一覧する
Miyoshi, Hideaki 著作を一覧する
Kuroda, Satoshi 著作を一覧する
Yasuda, Hiroshi 著作を一覧する
Kamiyama, Kenji 著作を一覧する
Nakagawara, Joji 著作を一覧する
Takigami, Masayoshi 著作を一覧する
Kondo, Takuma 著作を一覧する
Atsumi, Tatsuya 著作を一覧する
キーワード: Atherosclerosis
carotid artery disease
inflammation
macrophage
stroke
発行日: 2013年10月
出版者: Elsevier
誌名: Journal of stroke and cerebrovascular diseases
巻: 22
号: 7
開始ページ: 910
終了ページ: 918
出版社 DOI: 10.1016/j.jstrokecerebrovasdis.2012.11.020
抄録: Background: Proinflammatory (M1) macrophages and anti-inflammatory (M2) macrophages have been identified in atherosclerotic plaques. While these macrophages have been speculated to be related to plaque vulnerability, there are limited studies investigating this relationship. Therefore, we examined the association between macrophage phenotype (M1 versus M2) and plaque vulnerability and clinical events. Methods: Patients undergoing carotid endarterectomy received an ultrasound of the carotid artery before surgery. Plaques were processed for analysis by immunohistochemistry, Western blotting, and real-time polymerase chain reaction studies. Medical history and clinical data were obtained from medical records. Results: Patients were divided into 2 groups: those suffering from acute ischemic attack (symptomatic, n = 31) and those that did not present with symptoms (asymptomatic, n = 34). Ultrasound analysis revealed that plaque vulnerability was greater in the symptomatic group (P= .033; Chi-square test). Immunohistochemistry revealed that plaques from the symptomatic group had a greater concentration of M1 macrophages (CD68-, CD11c-positive) while plaques from the asymptomatic group had more M2 macrophages (CD163-positive). This observation was confirmed by Western blotting. Characterization by real-time polymerase chain reaction studies revealed that plaques from the symptomatic group had increased expression of the M1 markers CD68 and CD11c, as well as monocyte chemoattractive protein-1, interleukin-6, and matrix metalloproteinase-9. In addition, more M1 macrophages expressed in unstable plaques were defined by ultrasound analysis, while more M2 macrophages were expressed in stable plaques. Conclusions: Our data show that M1 macrophage content of atherosclerotic plaques is associated with clinical incidence of ischemic stroke and increased inflammation or fibrinolysis. We also show the benefits of using ultrasound to evaluate vulnerability in the plaques.
資料タイプ: article
URI: http://hdl.handle.net/2115/67265
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 三好 秀明

 

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