HUSCAP logo Hokkaido Univ. logo

Hokkaido University Collection of Scholarly and Academic Papers >
フード&メディカル イノベーション推進本部  >
雑誌発表論文等  >

Hypoxia-inducible ERO1α promotes cancer progression through modulation of integrin-β1 modification and signalling in HCT116 colorectal cancer cells

この資料はクリエイティブ・コモンズ・ライセンスの下で公開されています。

フルテキスト
s41598-017-09976-7.pdf3.71 MBPDF見る/開く
この文献へのリンクには次のURLを使用してください:http://hdl.handle.net/2115/67285

タイトル: Hypoxia-inducible ERO1α promotes cancer progression through modulation of integrin-β1 modification and signalling in HCT116 colorectal cancer cells
著者: Takei, Norio 著作を一覧する
Yoneda, Akihiro 著作を一覧する
Sakai-Sawada, Kaori 著作を一覧する
Kosaka, Marina 著作を一覧する
Minomi, Kenjiro 著作を一覧する
Tamura, Yasuaki 著作を一覧する
発行日: 2017年 8月24日
出版者: Nature Publishing Group
誌名: Scientific reports
巻: 7
開始ページ: 9389
出版社 DOI: 10.1038/s41598-017-09976-7
抄録: Endoplasmic reticulum disulphide oxidase 1α (ERO1α) is an oxidase localized in the endoplasmic reticulum that plays a role in the formation of disulphide bonds of secreted and cell-surface proteins. We previously showed that ERO1α is overexpressed in various types of cancer and we further identified ERO1α expression as a novel factor related to poor prognosis in cancer. However, the biological functions of ERO1α in cancer remain unclear. Here, we investigated the cell biological roles of ERO1α in the human colon-cancer cell line HCT116. ERO1α knockout (KO) by using CRISPR/Cas9 resulted in decreased tumourigenicity in vivo and reduced cell proliferation only under hypoxia in vitro, which suggested that ERO1α promotes cancer progression specifically in a low-oxygen environment. Thus, we evaluated the function of ERO1α in cell proliferation under hypoxia, and found that under hypoxic conditions, ERO1α KO resulted in a contact-inhibited morphology and diminished motility of cells. We further showed that ERO1α KO induced a change in integrin-β1 glycosylation and thus an attenuation of cell-surface integrin-β1 expression, which resulted in the aforementioned phenotype. Our study has established a previously unrecognized link between ERO1α expression and integrin activation, and thus provides new evidence for the effectiveness of ERO1α-targeted therapy for colorectal carcinoma.
資料タイプ: article
URI: http://hdl.handle.net/2115/67285
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 武井 則雄

 

本サイトに関するご意見・お問い合わせは repo at lib.hokudai.ac.jp へお願いします。 - 北海道大学