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Development of an Immunochromatography Assay (QuickNavi-Ebola) to Detect Multiple Species of Ebolaviruses

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Title: Development of an Immunochromatography Assay (QuickNavi-Ebola) to Detect Multiple Species of Ebolaviruses
Authors: Yoshida, Reiko Browse this author
Muramatsu, Shino Browse this author
Akita, Hiroshi Browse this author
Saito, Yuji Browse this author
Kuwahara, Miwa Browse this author
Kato, Daisuke Browse this author
Changula, Katendi Browse this author
Miyamoto, Hiroko Browse this author
Kajihara, Masahiro Browse this author
Manzoor, Rashid Browse this author
Furuyama, Wakako Browse this author
Marzi, Andrea Browse this author
Feldmann, Heinz Browse this author
Mweene, Aaron Browse this author
Masumu, Justin Browse this author
Kapeteshi, Jimmy Browse this author
Muyembe-Tamfum, Jean-Jacques Browse this author
Takada, Ayato Browse this author →KAKEN DB
Keywords: Ebola virus
monoclonal antibody
immunochromatography assay
Issue Date: 15-Oct-2016
Publisher: Oxford University Press
Journal Title: Journal of Infectious Diseases
Volume: 214
Issue: Suppl. 3
Start Page: S185
End Page: S191
Publisher DOI: 10.1093/infdis/jiw252
Abstract: The latest outbreak of Ebola virus disease (EVD) in West Africa has highlighted the urgent need for the development of rapid and reliable diagnostic assays. We used monoclonal antibodies specific to the ebolavirus nucleoprotein to develop an immunochromatography (IC) assay (QuickNavi-Ebola) for rapid diagnosis of EVD. The IC assay was first evaluated with tissue culture supernatants of infected Vero E6 cells and found to be capable of detecting 10(3)-10(4) focus-forming units/mL of ebolaviruses. Using serum samples from experimentally infected nonhuman primates, we confirmed that the assay could detect the viral antigen shortly after disease onset. It was also noted that multiple species of ebolaviruses could be detected by the IC assay. Owing to the simplicity of the assay procedure and absence of requirements for special equipment and training, QuickNavi-Ebola is expected to be a useful tool for rapid diagnosis of EVD.
Rights: This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of Infectious Diseases following peer review. The version of record 214(Suppl.3): S185-S191, 2016 is available online at:
Type: article (author version)
Appears in Collections:人獣共通感染症リサーチセンター (Research Center for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 高田 礼人

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