Title: | Development of an Immunochromatography Assay (QuickNavi-Ebola) to Detect Multiple Species of Ebolaviruses |
Authors: | Yoshida, Reiko Browse this author |
Muramatsu, Shino Browse this author |
Akita, Hiroshi Browse this author |
Saito, Yuji Browse this author |
Kuwahara, Miwa Browse this author |
Kato, Daisuke Browse this author |
Changula, Katendi Browse this author |
Miyamoto, Hiroko Browse this author |
Kajihara, Masahiro Browse this author |
Manzoor, Rashid Browse this author |
Furuyama, Wakako Browse this author |
Marzi, Andrea Browse this author |
Feldmann, Heinz Browse this author |
Mweene, Aaron Browse this author |
Masumu, Justin Browse this author |
Kapeteshi, Jimmy Browse this author |
Muyembe-Tamfum, Jean-Jacques Browse this author |
Takada, Ayato Browse this author →KAKEN DB |
Keywords: | Ebola virus |
ebolavirus |
EVD |
filovirus |
nucleoprotein |
monoclonal antibody |
immunochromatography assay |
diagnosis |
Issue Date: | 15-Oct-2016 |
Publisher: | Oxford University Press |
Journal Title: | Journal of Infectious Diseases |
Volume: | 214 |
Issue: | Suppl. 3 |
Start Page: | S185 |
End Page: | S191 |
Publisher DOI: | 10.1093/infdis/jiw252 |
Abstract: | The latest outbreak of Ebola virus disease (EVD) in West Africa has highlighted the urgent need for the development of rapid and reliable diagnostic assays. We used monoclonal antibodies specific to the ebolavirus nucleoprotein to develop an immunochromatography (IC) assay (QuickNavi-Ebola) for rapid diagnosis of EVD. The IC assay was first evaluated with tissue culture supernatants of infected Vero E6 cells and found to be capable of detecting 10(3)-10(4) focus-forming units/mL of ebolaviruses. Using serum samples from experimentally infected nonhuman primates, we confirmed that the assay could detect the viral antigen shortly after disease onset. It was also noted that multiple species of ebolaviruses could be detected by the IC assay. Owing to the simplicity of the assay procedure and absence of requirements for special equipment and training, QuickNavi-Ebola is expected to be a useful tool for rapid diagnosis of EVD. |
Rights: | This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of Infectious Diseases following peer review. The version of record 214(Suppl.3): S185-S191, 2016 is available online at: https://doi.org/10.1093/infdis/jiw252. |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/67314 |
Appears in Collections: | 人獣共通感染症国際共同研究所 (International Institute for Zoonosis Control) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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