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Hokkaido University Collection of Scholarly and Academic Papers >
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北海道歯学雑誌 >
第38巻 記念特集号 >
Bone regeneration around N-acetyl cysteine-loaded nanotube titanium dental implants
| Title: | Bone regeneration around N-acetyl cysteine-loaded nanotube titanium dental implants |
| Authors: | Lee, Young-Hee Browse this author | | Bhattarai, Govinda Browse this author | | Park, Il-Song Browse this author | | Kim, Ga-Ram Browse this author | | Kim, Go-Eun Browse this author | | Lee, Min-Ho Browse this author | | Kim, In-Ju Browse this author | | Seo, Jae-Min Browse this author | | Ahn, Seung-Geun Browse this author | | Yi, Ho-Keun Browse this author |
| Keywords: | nanotubes | | NAC | | dental implant | | drug delivery | | osseointegration |
| Issue Date: | Sep-2017 |
| Publisher: | 北海道歯学会 |
| Journal Title: | 北海道歯学雑誌 |
| Volume: | 38 |
| Issue: | Special issue |
| Start Page: | 9 |
| End Page: | 19 |
| Abstract: | New strategies involving drugs loading onto implant surfaces are required to enhance osseointegration and shorten healing time after implantation. In this study, we examined the feasibility of N -acetyl cysteine (NAC)-loaded nanotube titanium (NLN-Ti) implants as a potential drug delivery system. To determine the effect of NLN-Ti in in vitro and in vivo, viability and ROS formation was assessed and enzyme-linked immunosorbant assay (ELISA), Western blot, micro-computed tomography (μ-CT), hematoxylin and eoxin (H&E) staining and immunohistochemical (IHC) analysis were performed. In vitro, cell viability was increased and inflammatory responses and reduced oxidative stress-related defense were decreased with MC 3T3-E1 cells exposed to a sustained release of NAC from NLN-Ti implants. Following NLN-Ti implant installation, μ-CT and histomorphometric analysis revealed an increase of newly formed bone volume and bone mineral density in the mandibles of Sprague Dawley rats and beagle dogs. Relatively well-formed new bone was demonstrated in close contact to the NLN-Ti implant surface by H&E staining. IHC revealed a significantly higher expression of bone morphogenetic protein-2, -7 and heme oxygenase-1, and reduced the expression of the receptor activator of nuclear factor-kappa B ligand. The data indicate that NLN-Ti implants enhance osseointegration and highlight the value of the animal model in assessing diverse biological responses to dental implants. |
| Type: | article |
| URI: | http://hdl.handle.net/2115/67331 |
| Appears in Collections: | 北海道歯学雑誌 > 第38巻 記念特集号
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