| Title: | Characterization of a thermophilic 4-O-β-d-mannosyl-d-glucose phosphorylase fromRhodothermus marinus |
| Authors: | Jaito, Nongluck Browse this author |
| Saburi, Wataru Browse this author →KAKEN DB |
| Odaka, Rei Browse this author |
| Kido, Yusuke Browse this author |
| Hamura, Ken Browse this author |
| Nishimoto, Mamoru Browse this author |
| Kitaoka, Motomitsu Browse this author |
| Matsui, Hirokazu Browse this author →KAKEN DB |
| Mori, Haruhide Browse this author →KAKEN DB |
| Keywords: | Rhodothermus marinus |
| 4-O-β-d-mannosyl-d-glucose phosphorylase |
| mannan |
| substrate specificity |
| phosphorolysis |
| Issue Date: | 2014 |
| Publisher: | Taylor & Francis |
| Journal Title: | Bioscience, Biotechnology, and Biochemistry |
| Volume: | 78 |
| Issue: | 2 |
| Start Page: | 263 |
| End Page: | 270 |
| Publisher DOI: | 10.1080/09168451.2014.882760 |
| PMID: | 25036679 |
| Abstract: | 4-O-β-d-Mannosyl-d-glucose phosphorylase (MGP), found in anaerobes, converts 4-O-β-d-mannosyl-d-glucose (Man-Glc) to α-d-mannosyl phosphate and d-glucose. It participates in mannan metabolism with cellobiose 2-epimerase (CE), which converts β-1,4-mannobiose to Man-Glc. A putative MGP gene is present in the genome of the thermophilic aerobe Rhodothermus marinus (Rm) upstream of the gene encoding CE. Konjac glucomannan enhanced production by R. marinus of MGP, CE, and extracellular mannan endo-1,4-β-mannosidase. Recombinant RmMGP catalyzed the phosphorolysis of Man-Glc through a sequential bi–bi mechanism involving ternary complex formation. Its molecular masses were 45 and 222 kDa under denaturing and nondenaturing conditions, respectively. Its pH and temperature optima were 6.5 and 75 °C, and it was stable between pH 5.5–8.3 and below 80 °C. In the reverse reaction, RmMGP had higher acceptor preferences for 6-deoxy-d-glucose and d-xylose than R. albus NE1 MGP. In contrast to R. albus NE1 MGP, RmMGP utilized methyl β-d-glucoside and 1,5-anhydro-d-glucitol as acceptor substrates. |
| Rights: | This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience biotechnology and biochemistry on 2014, available online: http://wwww.tandfonline.com/10.1080/09168451.2014.882760 |
| Type: | article (author version) |
| URI: | http://hdl.handle.net/2115/67498 |
| Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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