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Biochemical Characterization of a Thermophilic Cellobiose 2-Epimerase from a Thermohalophilic Bacterium,Rhodothermus marinusJCM9785
Title: | Biochemical Characterization of a Thermophilic Cellobiose 2-Epimerase from a Thermohalophilic Bacterium,Rhodothermus marinusJCM9785 |
Authors: | Ojima, Teruyo Browse this author | Saburi, Wataru Browse this author →KAKEN DB | Sato, Hiroki Browse this author | Yamamoto, Takeshi Browse this author | Mori, Haruhide Browse this author →KAKEN DB | Matsui, Hirokazu Browse this author →KAKEN DB |
Keywords: | cellobiose 2-epimerase | epilactose | Rhodothermus marinus | N-acetylglucosamine 2-epimerase |
Issue Date: | Nov-2011 |
Publisher: | Taylor & Francis |
Journal Title: | Bioscience, Biotechnology, and Biochemistry |
Volume: | 75 |
Issue: | 11 |
Start Page: | 2162 |
End Page: | 2168 |
Publisher DOI: | 10.1271/bbb.110456 |
PMID: | 22056431 |
Abstract: | Cellobiose 2-epimerase (CE) reversibly converts glucose residue to mannose residue at the reducing end of β-1,4-linked oligosaccharides. It efficiently produces epilactose carrying prebiotic properties from lactose, but the utilization of known CEs is limited due to thermolability. We focused on thermoholophilic Rhodothermus marinus JCM9785 as a CE producer, since a CE-like gene was found in the genome of R. marinus DSM4252. CE activity was detected in the cell extract of R. marinus JCM9785. The deduced amino acid sequence of the CE gene from R. marinus JCM9785 (RmCE) was 94.2% identical to that from R. marinus DSM4252. The N-terminal amino acid sequence and tryptic peptide masses of the native enzyme matched those of RmCE. The recombinant RmCE was most active at 80 °C at pH 6.3, and stable in a range of pH 3.2–10.8 and below 80 °C. In contrast to other CEs, RmCE demonstrated higher preference for lactose over cellobiose. |
Rights: | This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience biotechnology and biochemistry on 11/2011, available online: http://wwww.tandfonline.com/10.1271/bbb.110456 |
Type: | article (author version) |
URI: | http://hdl.handle.net/2115/67501 |
Appears in Collections: | 農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 佐分利 亘
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