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Biochemical Characterization of a Thermophilic Cellobiose 2-Epimerase from a Thermohalophilic Bacterium,Rhodothermus marinusJCM9785

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Title: Biochemical Characterization of a Thermophilic Cellobiose 2-Epimerase from a Thermohalophilic Bacterium,Rhodothermus marinusJCM9785
Authors: Ojima, Teruyo Browse this author
Saburi, Wataru Browse this author →KAKEN DB
Sato, Hiroki Browse this author
Yamamoto, Takeshi Browse this author
Mori, Haruhide Browse this author →KAKEN DB
Matsui, Hirokazu Browse this author →KAKEN DB
Keywords: cellobiose 2-epimerase
epilactose
Rhodothermus marinus
N-acetylglucosamine 2-epimerase
Issue Date: Nov-2011
Publisher: Taylor & Francis
Journal Title: Bioscience, Biotechnology, and Biochemistry
Volume: 75
Issue: 11
Start Page: 2162
End Page: 2168
Publisher DOI: 10.1271/bbb.110456
PMID: 22056431
Abstract: Cellobiose 2-epimerase (CE) reversibly converts glucose residue to mannose residue at the reducing end of β-1,4-linked oligosaccharides. It efficiently produces epilactose carrying prebiotic properties from lactose, but the utilization of known CEs is limited due to thermolability. We focused on thermoholophilic Rhodothermus marinus JCM9785 as a CE producer, since a CE-like gene was found in the genome of R. marinus DSM4252. CE activity was detected in the cell extract of R. marinus JCM9785. The deduced amino acid sequence of the CE gene from R. marinus JCM9785 (RmCE) was 94.2% identical to that from R. marinus DSM4252. The N-terminal amino acid sequence and tryptic peptide masses of the native enzyme matched those of RmCE. The recombinant RmCE was most active at 80 °C at pH 6.3, and stable in a range of pH 3.2–10.8 and below 80 °C. In contrast to other CEs, RmCE demonstrated higher preference for lactose over cellobiose.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience biotechnology and biochemistry on 11/2011, available online: http://wwww.tandfonline.com/10.1271/bbb.110456
Type: article (author version)
URI: http://hdl.handle.net/2115/67501
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐分利 亘

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