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Physiological and Biochemical Characterization of Three Nucleoside Diphosphate Kinase Isozymes from Rice (Oryza sativaL.)

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Title: Physiological and Biochemical Characterization of Three Nucleoside Diphosphate Kinase Isozymes from Rice (Oryza sativaL.)
Authors: Kihara, Akihiko Browse this author
Saburi, Wataru Browse this author →KAKEN DB
Wakuta, Shinji Browse this author
Kim, Myung-Hee Browse this author
Hamada, Shigeki Browse this author
Ito, Hiroyuki Browse this author
Imai, Ryozo Browse this author
Matsui, Hirokazu Browse this author →KAKEN DB
Keywords: nucleoside diphosphate kinase
subcellular localization
ping-pong mechanism
gene expression
enzyme characteristics
Issue Date: Sep-2011
Publisher: Taylor & Francis
Journal Title: Bioscience, Biotechnology, and Biochemistry
Volume: 75
Issue: 9
Start Page: 1740
End Page: 1745
Publisher DOI: 10.1271/bbb.110285
PMID: 21897044
Abstract: Nucleoside diphosphate kinase (NDPK) is a ubiquitous enzyme that catalyzes the transfer of the γ-phosphoryl group from a nucleoside triphosphate to a nucleoside diphosphate. In this study, we examined the subcellular localization, tissue-specific gene expression, and enzymatic characteristics of three rice NDPK isozymes (OsNDPK1-OsNDPK3). Sequence comparison of the three OsNDPKs suggested differential subcellular localization. Transient expression of green fluorescence protein-fused proteins in onion cells indicated that OsNDPK2 and OsNDPK3 are localized to plastid and mitochondria respectively, while OsNDPK1 is localized to the cytosol. Expression analysis indicated that all the OsNDPKs are expressed in the leaf, leaf sheath, and immature seeds, except for OsNDPK1, in the leaf sheath. Recombinant OsNDPK2 and OsNDPK3 showed lower optimum pH and higher stability under acidic pH than OsNDPK1. In ATP formation, all the OsNDPKs displayed lower K(m) values for the second substrate, ADP, than for the first substrate, NTP, and showed lowest and highest K(m) values for GTP and CTP respectively.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on 2011 Sep, available online: http://wwww.tandfonline.com/10.1271/bbb.110285.
Type: article (author version)
URI: http://hdl.handle.net/2115/67570
Appears in Collections:農学院・農学研究院 (Graduate School of Agriculture / Faculty of Agriculture) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 佐分利 亘

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