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Physiological and Biochemical Characterization of Three Nucleoside Diphosphate Kinase Isozymes from Rice (Oryza sativaL.)

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タイトル: Physiological and Biochemical Characterization of Three Nucleoside Diphosphate Kinase Isozymes from Rice (Oryza sativaL.)
著者: Kihara, Akihiko 著作を一覧する
Saburi, Wataru 著作を一覧する
Wakuta, Shinji 著作を一覧する
Kim, Myung-Hee 著作を一覧する
Hamada, Shigeki 著作を一覧する
Ito, Hiroyuki 著作を一覧する
Imai, Ryozo 著作を一覧する
Matsui, Hirokazu 著作を一覧する
キーワード: nucleoside diphosphate kinase
subcellular localization
ping-pong mechanism
gene expression
enzyme characteristics
発行日: 2011年 9月
出版者: Taylor & Francis
誌名: Bioscience, Biotechnology, and Biochemistry
巻: 75
号: 9
開始ページ: 1740
終了ページ: 1745
出版社 DOI: 10.1271/bbb.110285
抄録: Nucleoside diphosphate kinase (NDPK) is a ubiquitous enzyme that catalyzes the transfer of the γ-phosphoryl group from a nucleoside triphosphate to a nucleoside diphosphate. In this study, we examined the subcellular localization, tissue-specific gene expression, and enzymatic characteristics of three rice NDPK isozymes (OsNDPK1-OsNDPK3). Sequence comparison of the three OsNDPKs suggested differential subcellular localization. Transient expression of green fluorescence protein-fused proteins in onion cells indicated that OsNDPK2 and OsNDPK3 are localized to plastid and mitochondria respectively, while OsNDPK1 is localized to the cytosol. Expression analysis indicated that all the OsNDPKs are expressed in the leaf, leaf sheath, and immature seeds, except for OsNDPK1, in the leaf sheath. Recombinant OsNDPK2 and OsNDPK3 showed lower optimum pH and higher stability under acidic pH than OsNDPK1. In ATP formation, all the OsNDPKs displayed lower K(m) values for the second substrate, ADP, than for the first substrate, NTP, and showed lowest and highest K(m) values for GTP and CTP respectively.
Rights: This is an Accepted Manuscript of an article published by Taylor & Francis in Bioscience, Biotechnology, and Biochemistry on 2011 Sep, available online: http://wwww.tandfonline.com/10.1271/bbb.110285.
資料タイプ: article (author version)
URI: http://hdl.handle.net/2115/67570
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 佐分利 亘

 

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