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Putative endogenous filovirus VP35-like protein potentially functions as an IFN antagonist but not a polymerase cofactor


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タイトル: Putative endogenous filovirus VP35-like protein potentially functions as an IFN antagonist but not a polymerase cofactor
著者: Kondoh, Tatsunari 著作を一覧する
Manzoor, Rashid 著作を一覧する
Nao, Naganori 著作を一覧する
Maruyama, Junki 著作を一覧する
Furuyama, Wakako 著作を一覧する
Miyamoto, Hiroko 著作を一覧する
Shigeno, Asako 著作を一覧する
Kuroda, Makoto 著作を一覧する
Matsuno, Keita 著作を一覧する
Fujikura, Daisuke 著作を一覧する
Kajihara, Masahiro 著作を一覧する
Yoshida, Reiko 著作を一覧する
Igarashi, Manabu 著作を一覧する
Takada, Ayato 著作を一覧する
発行日: 2017年10月17日
出版者: PLOS
誌名: PLoS ONE
巻: 12
号: 10
開始ページ: 1
終了ページ: 17
出版社 DOI: 10.1371/journal.pone.0186450
抄録: It has been proposed that some non-retroviral RNA virus genes are integrated into vertebrate genomes. Endogenous filovirus-like elements (EFLs) have been discovered in some mammalian genomes. However, their potential roles in ebolavirus infection are unclear. A filovirus VP35-like element (mlEFL35) is found in the little brown bat (Myotis lucifugus) genome. Putative mlEFL35-derived protein (mlEFL35p) contains nearly full-length amino acid sequences corresponding to ebolavirus VP35. Ebola virus VP35 has been shown to bind double-stranded RNA, leading to inhibition of type I interferon (IFN) production, and is also known as a viral polymerase cofactor that is essential for viral RNA transcription/replication. In this study, we transiently expressed mlEFL35p in human kidney cells and investigated its biological functions. We first found that mlEFL35p was coimmunoprecipitated with itself and ebolavirus VP35s but not with the viral nucleoprotein. Then the biological functions of mlEFL35p were analyzed by comparing it to ebolavirus VP35s. We found that the expression of mlEFL35p significantly inhibited human IFN-beta promoter activity as well as VP35s. By contrast, expression of mlEFL35p did not support viral RNA transcription/replication and indeed slightly decrease the reporter gene expression in a minigenome assay. These results suggest that mlEFL35p potentially acts as an IFN antagonist but not a polymerase cofactor.
資料タイプ: article
出現コレクション:雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

提供者: 高田 礼人


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