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Cigarette smoking alters sialylation in the Fallopian tube of women, with implications for the pathogenesis of ectopic pregnancy

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Title: Cigarette smoking alters sialylation in the Fallopian tube of women, with implications for the pathogenesis of ectopic pregnancy
Authors: Nio-Kobayashi, Junko Browse this author →KAKEN DB
Abidin, Hazirah B. Z. Browse this author
Brown, Jeremy K. Browse this author
Iwanaga, Toshihiko Browse this author →KAKEN DB
Horne, Andrew W. Browse this author
Duncan, W. Colin Browse this author
Keywords: sialic acid
sialyltransferases
tubal ectopic pregnancy
cigarette smoking
cilia
galectin
Issue Date: Dec-2016
Publisher: Wiley
Journal Title: Molecular Reproduction and Development
Volume: 83
Issue: 12
Start Page: 1083
End Page: 1091
Publisher DOI: 10.1002/mrd.22747
PMID: 27704662
Abstract: The expression of sialyltransferases decreases during tubal ectopic pregnancy in women and cigarette smoking alters the expression of ST6GAL1 and ST3GAL5, potentially resulting in a decreased tubal transport and an increased receptivity for blastocysts.
We recently reported potential involvement of galectin-1 and galectin-3, β-galactoside-binding lectins, in pathogenesis of tubal ectopic pregnancy. However, the precise role of galectins and their ligand glycoconjugates remain unclear. Sialylation serves surface negativity to block blastocyst implantation, and α2,6-sialylation on terminal galactose catalyzed by a sialyltransferase, ST6GAL1, inhibits the binding of galectin-1, which is increased in expression level during tubal ectopic implantation. We here investigated the expression of α2,3- and α2,6-galactoside sialyltransferases (ST3GAL1-6 and ST6GAL1-2) and the localization of sialic acids in the Fallopian tube from women with or without ectopic implantation. The expression level of ST6GAL1 was higher in the mid-secretory phase than the proliferative phase (P<0.0001) of non-pregnant women. In the Fallopian tube with ectopic implantation, ST6GAL1 was lower (P<0.0001) as were ST3GAL3 (P=0.0029), ST3GAL5 (P=0.0089), and ST3GAL6 (P=0.0018). Cigarette smoking, a major risk factor for tubal ectopic pregnancy, was associated with a reduced mid-secretory phase expression of ST6GAL1 (P=0.0298) and elevated expression of ST3GAL5 (P=0.0006), an enzyme known to be involved in ciliogenesis. Both α2,3- and α2,6-sialic acids were localized to the surface of the Fallopian tube epithelium. Sialic acid-containing ciliated inclusion cysts, which are known to be associated with abnormal ciliogenesis, were observed within the epithelium, and the number of cysts increased (P=0.0177) in women who smoked, suggesting that abnormal ciliogenesis is associated with smoking. These results suggest that cigarette smoking alters sialylation in the Fallopian tube epithelium and potentially involved in a decreased tubal transport and an increased receptivity for blastocyst in human Fallopian tube.
Rights: This is the peer reviewed version of the following article: Nio-Kobayashi, J., Abidin, H. B.Z., Brown, J. K., Iwanaga, T., Horne, A. W. and Duncan, W. C. (2016), Cigarette smoking alters sialylation in the Fallopian tube of women, with implications for the pathogenesis of ectopic pregnancy. Mol. Reprod. Dev., 83: 1083–1091, which has been published in final form at http://dx.doi.org/10.1002/mrd.22747. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Type: article (author version)
URI: http://hdl.handle.net/2115/67737
Appears in Collections:医学院・医学研究院 (Graduate School of Medicine / Faculty of Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 小林 純子

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