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Crystal structure of the flexible tandem repeat domain of bacterial cellulose synthesis subunit C

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Title: Crystal structure of the flexible tandem repeat domain of bacterial cellulose synthesis subunit C
Authors: Nojima, Shingo Browse this author
Fujishima, Ayumi Browse this author
Kato, Koji Browse this author
Ohuchi, Kayoko Browse this author
Shimizu, Nobutaka Browse this author
Yonezawa, Kento Browse this author
Tajima, Kenji Browse this author
Yao, Min Browse this author →KAKEN DB
Issue Date: 12-Oct-2017
Publisher: Nature Publishing Group
Journal Title: Scientific reports
Volume: 7
Start Page: 13018
Publisher DOI: 10.1038/s41598-017-12530-0
Abstract: Bacterial cellulose (BC) is synthesized and exported through the cell membrane via a large protein complex (terminal complex) that consists of three or four subunits. BcsC is a little-studied subunit considered to export BC to the extracellular matrix. It is predicted to have two domains: a tetratrico peptide repeat (TPR) domain and a beta-barrelled outer membrane domain. Here we report the crystal structure of the N-terminal part of BcsC-TPR domain (Asp24-Arg272) derived from Enterobacter CJF-002. Unlike most TPR-containing proteins which have continuous TPR motifs, this structure has an extra a-helix between two clusters of TPR motifs. Five independent molecules in the crystal had three different conformations that varied at the hinge of the inserted a-helix. Such structural feature indicates that the inserted a-helix confers flexibility to the chain and changes the direction of the TPR super-helix, which was also suggested by structural analysis of BcsC-TPR (Asp24-Leu664) in solution by size exclusion chromatography-small-angle X-ray scattering. The flexibility at the a-helical hinge may play important role for exporting glucan chains.
Rights: http://creativecommons.org/licenses/by/4.0/
Type: article
URI: http://hdl.handle.net/2115/67750
Appears in Collections:生命科学院・先端生命科学研究院 (Graduate School of Life Science / Faculty of Advanced Life Science) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 姚 閔

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