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Hokkaido University Collection of Scholarly and Academic Papers >
Graduate School of Dental Medicine / Faculty of Dental Medicine >
Peer-reviewed Journal Articles, etc >
Antibacterial and cytotoxic effects of photoexcited Au clusters via blue high-power or white low-power light emitting diode irradiation
This item is licensed under:Creative Commons Attribution 4.0 International
| Title: | Antibacterial and cytotoxic effects of photoexcited Au clusters via blue high-power or white low-power light emitting diode irradiation |
| Authors: | Miyata, Saori Browse this author | | Miyaji, Hirofumi Browse this author →KAKEN DB | | Kawasaki, Hideya Browse this author | | Nishida, Erika Browse this author | | Shitomi, Kanako Browse this author | | Akasaka, Tsukasa Browse this author →KAKEN DB | | Tanaka, Saori Browse this author →KAKEN DB | | Iizuka, Tadashi Browse this author | | Sugaya, Tsutomu Browse this author →KAKEN DB |
| Keywords: | Antimicrobial photodynamic therapy | | captopril-protected gold (Au) clusters | | lysozyme-stabilized gold clusters | | photosensitizer | | Streptococcus mutans |
| Issue Date: | Oct-2017 |
| Publisher: | Open Access Text |
| Journal Title: | Biology, Engineering and Medicine |
| Volume: | 2 |
| Issue: | 4 |
| Publisher DOI: | 10.15761/BEM.1000126 |
| Abstract: | The development of photosensitizers and light sources has enabled the use of antimicrobial photodynamic therapy (aPDT) in various dental therapies. In the present study, we compared the antibacterial and cytotoxic effects of Au clusters photoexcited by blue and white LED irradiation. We fabricated novel photosensitizers, captopril-protected gold (Capt-Au) clusters and lysozyme-stabilized gold (Lyz-Au) clusters, for aPDT. Au clusters were then photoexcited by two kinds of light sources, blue high-power and white low-power light-emitting diodes (LEDs). Since white LED contains a wide spectrum of light (400–750 nm), white LED would be relevant for aPDT even if using a low-power source. The turbidity and viability of Streptococcus mutans were assessed following application of Capt-Au clusters (500 μg/mL) or Lyz-Au clusters (1,000 μg/mL) photoexcited by a blue high-power LED (1,000 mW/cm2) or white low-power LED (80 mW/cm2). In addition, the cytotoxicity of Au clusters and LED irradiation was evaluated in NIH3T3 and MC3T3-E1 cells. Au clusters photoexcited by the white low-power LED equally decreased the turbidity and viability of S. mutans compared with blue high-power LED. However, Au clusters photoexcited by white LED irradiation caused decreased cytotoxicity in mammalian cells compared with those photoexcited by blue LED irradiation. In conclusion, white LEDs possess biosafe properties for aPDT using Au clusters. |
| Rights: | http://creativecommons.org/licenses/by/4.0/ |
| Type: | article |
| URI: | http://hdl.handle.net/2115/67756 |
| Appears in Collections: | 歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)
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Submitter: 宮治 裕史
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