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Antibacterial and cytotoxic effects of photoexcited Au clusters via blue high-power or white low-power light emitting diode irradiation

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Title: Antibacterial and cytotoxic effects of photoexcited Au clusters via blue high-power or white low-power light emitting diode irradiation
Authors: Miyata, Saori Browse this author
Miyaji, Hirofumi Browse this author →KAKEN DB
Kawasaki, Hideya Browse this author
Nishida, Erika Browse this author
Shitomi, Kanako Browse this author
Akasaka, Tsukasa Browse this author →KAKEN DB
Tanaka, Saori Browse this author →KAKEN DB
Iizuka, Tadashi Browse this author
Sugaya, Tsutomu Browse this author →KAKEN DB
Keywords: Antimicrobial photodynamic therapy
captopril-protected gold (Au) clusters
lysozyme-stabilized gold clusters
Streptococcus mutans
Issue Date: Oct-2017
Publisher: Open Access Text
Journal Title: Biology, Engineering and Medicine
Volume: 2
Issue: 4
Publisher DOI: 10.15761/BEM.1000126
Abstract: The development of photosensitizers and light sources has enabled the use of antimicrobial photodynamic therapy (aPDT) in various dental therapies. In the present study, we compared the antibacterial and cytotoxic effects of Au clusters photoexcited by blue and white LED irradiation. We fabricated novel photosensitizers, captopril-protected gold (Capt-Au) clusters and lysozyme-stabilized gold (Lyz-Au) clusters, for aPDT. Au clusters were then photoexcited by two kinds of light sources, blue high-power and white low-power light-emitting diodes (LEDs). Since white LED contains a wide spectrum of light (400–750 nm), white LED would be relevant for aPDT even if using a low-power source. The turbidity and viability of Streptococcus mutans were assessed following application of Capt-Au clusters (500 μg/mL) or Lyz-Au clusters (1,000 μg/mL) photoexcited by a blue high-power LED (1,000 mW/cm2) or white low-power LED (80 mW/cm2). In addition, the cytotoxicity of Au clusters and LED irradiation was evaluated in NIH3T3 and MC3T3-E1 cells. Au clusters photoexcited by the white low-power LED equally decreased the turbidity and viability of S. mutans compared with blue high-power LED. However, Au clusters photoexcited by white LED irradiation caused decreased cytotoxicity in mammalian cells compared with those photoexcited by blue LED irradiation. In conclusion, white LEDs possess biosafe properties for aPDT using Au clusters.
Type: article
Appears in Collections:歯学院・歯学研究院 (Graduate School of Dental Medicine / Faculty of Dental Medicine) > 雑誌発表論文等 (Peer-reviewed Journal Articles, etc)

Submitter: 宮治 裕史

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